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重要文件

C1403

Sigma-Aldrich

胆固醇酯酶 来源于假单胞菌

lyophilized powder, ≥200,000 units/g protein

同義詞:

胆固醇酯酶 来源于荧光假单胞菌, 甾醇酯酰水解酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

比活性

≥200,000 units/g protein

分子量

~300 kDa

成份

protein, ≥40% biuret

儲存溫度

−20°C

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應用

当在临床分析中与胆固醇氧化酶结合使用时,该酶可用于酶法测定总胆固醇。

生化/生理作用

胆固醇酯酶(CE)是一种可逆酶,可以水解或合成胆固醇和其他固醇的脂肪酸酯。水不溶性长链脂肪酸酯的水解需要经过胆盐活化。短链脂肪酸和溶血磷脂的水溶性酯的水解则不需要胆盐的活化。它还可水解三、二和单酰基甘油、磷脂、溶血磷脂和神经酰胺。该酶在脂质和脂蛋白代谢以及动脉粥样硬化中可能具有多种功能。

物理性質

稳定性:在–20°C稳定至少一年
等电点:5.9 ± 0.1
米氏常数:5.4 x 10‾5M(亚油酸脂),6.6 x 10‾5M(油酸)
3.7 x 10‾5M(亚油酸脂),1.5 x 10‾4M(棕榈酸)
1.2 x 10‾4M(肉豆蔻),2.3 x 10‾5M(硬脂酸酯)
抑制剂:Hg++、Ag+、离子型去垢剂
最适pH值: 7.0 − 9.0
最适温度:40°C
pH稳定性: pH 5.0 − 9.0 (25°C, 24hr)
热稳定性:低于55°C(pH 7.5,10分钟)

單位定義

在牛磺胆酸盐存在下,一个单位在 pH7.0,37°C 下每分钟将 1.0μmole胆固醇油酸酯水解成胆固醇和油酸。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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David Y Hui et al.
Journal of lipid research, 43(12), 2017-2030 (2002-11-28)
Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally
Kevin R Shreder et al.
Bioorganic & medicinal chemistry letters, 22(17), 5748-5751 (2012-08-11)
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Pornpen Srisawasdi et al.
Journal of clinical laboratory analysis, 26(6), 420-430 (2012-11-13)
Accurate determination of cholesterol requires complete hydrolysis of cholesteryl esters and must be very fast for the kinetic cholesterol assay. We investigated the properties of cholesterol esterase derived from Pseudomonas fluorescens, Candida cylindracea, bovine pancreas, and porcine pancreas for cholesterol
Farid Shokry Ataya
Molecules (Basel, Switzerland), 17(9), 10399-10413 (2012-09-01)
Acid lipase belongs to a family of enzymes that is mainly present in lysosomes of different organs and the stomach. It is characterized by its capacity to withstand acidic conditions while maintaining high lipolytic activity. We cloned for the first
Vincent Delorme et al.
PloS one, 7(9), e46493-e46493 (2012-10-03)
Lipid metabolism plays an important role during the lifetime of Mycobacterium tuberculosis, the causative agent of tuberculosis. Although M. tuberculosis possesses numerous lipolytic enzymes, very few have been characterized yet at a biochemical/pharmacological level. This study was devoted to the

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