推薦產品
應用
梭菌蛋白酶(源于溶组织梭菌)已用作灌注液的蛋白水解酶,检测其对管中血细胞比容(tube hematocrit)的影响。还用于T4噬菌体(RB69 gp43)DNA聚合酶(gp43)的限制性分解。
生化/生理作用
梭菌蛋白酶,来自溶组织梭菌,由两条分子量各为41.7 kDa和15.4 kDa的肽链组成。梭菌蛋白酶对Arg-Xaa肽键具有高限制性的底物特异性。因此,梭菌蛋白酶可服务于蛋白测序。据研究,它也可作为催化剂,用于缩合含Arg-Pro肽键的重要药物肽。
包裝
无底玻璃瓶。内含物装在锥底内插管中。
品質
纯化制品,基本上不含盐
單位定義
25 °C、pH 7.6,在2.5 mM DTT存在条件下,一个单位每分钟水解1.0μmole BAEE。
分析報告
本品在使用前,要先溶于含1.0 mM乙酸钙的2.5 mM二硫苏糖醇中活化2-3小时。
抑制劑
產品號碼
描述
訂價
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
Journal of biotechnology, 131(3), 346-352 (2007-09-05)
In this study, the clostripain gene was modified and its signal sequence was replaced with that of penicillin G acylase (PGA). The core clostripain protein fused to the PGA signal peptide was also prepared. With regard to the expression of
Journal of medicinal chemistry, 47(8), 1889-1892 (2004-04-02)
Aza-peptide Michael acceptors are a new class of irreversible inhibitors that are highly potent and specific for clan CD cysteine proteases. The aza-Asp derivatives were specific for caspases, while aza-Asn derivatives were effective legumain inhibitors. Aza-Lys and aza-Orn derivatives were
European journal of biochemistry, 271(5), 983-992 (2004-03-11)
In this study we investigate the active-site structure and the catalytic mechanism of clostripain by using a combination of three separate techniques: affinity labelling, site-directed mutagenesis and molecular modelling. A benzamidinyl-diazo dichlorotriazine dye (BDD) was shown to act as an
Antibacterial activity of casein-derived peptides isolated from rabbit (Oryctolagus cuniculus) milk.
The Journal of dairy research, 70(2), 189-197 (2003-06-13)
Acid-precipitated rabbit 'whole casein' was digested by trypsin, chymotrypsin, pepsin, and clostripain to screen for possible peptides with antibacterial properties. The peptide fragments were separated by reversed-phase chromatography. The collected fractions were pooled and their antibacterial properties tested against Escherichia
Synthesis of neo-peptidoglycans: an unexpected activity of proteases.
Angewandte Chemie (International ed. in English), 41(15), 2735-2738 (2002-08-31)
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