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重要文件

SCR103

Sigma-Aldrich

I型胶原酶

The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.

同義詞:

Collagenase Type I from Clostridium histolyticum, Tissue dissociation

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About This Item

分類程式碼代碼:
12352204
eCl@ss:
32160801
NACRES:
NA.75

品質等級

形狀

powder

製造商/商標名

Chemicon®

技術

cell culture | stem cell: suitable

輸入

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

運輸包裝

wet ice

一般說明

I 型胶原酶(来自溶组织梭状芽胞杆菌)是一种粗制的胶原酶制剂,可用于分离原代细胞或通过酶促方法进行组织分离。该制剂还可能含有酪蛋白酶、梭菌蛋白酶和胰蛋白酶活性。该制剂可用于分离培养中的细胞,并已成功用于传代用 Millipore 的 HEScGROTM 无动物成分培养基培养的人胚胎干细胞。

包裝

1 小瓶包含 250mg 冻干粉

品質

通过改良的 Mandl(1964)来测定每批 I 型胶原酶,并使用 Moore and Stein(1948)的比色茚三酮法测定胶原蛋白分解的程度。然后测试每批在 Millipore 的 HEScGRO hES 细胞培养基中培养的人胚胎干细胞成功传代的情况。经过五次传代后,对细胞进行合适的标志物表达和形态学表征。

外觀

I 型胶原酶以透析的冻干粉形式提供。

儲存和穩定性

I 型胶原酶应以粉末形式在 2-8oC 下保存,直到可以使用为止。可以将 I 型胶原酶溶液等分并保存在 -20oC,但不应反复冻融。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

仅供研究使用。在法国,在用于科研目的时(包括进口和出口活动(《公共健康法》第L 1211-1条,第2节))该产品为管制品。购买者(即最终用户)需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Astaxanthin (AX) a marine carotenoid is a powerful natural antioxidant which protects against oxidative stress and improves muscle performance. Retinol and its derivatives were described to affect lipid and energy metabolism. Up to date, the effects of AX and retinol
Fengqin Fang et al.
Cell reports, 37(6), 109981-109981 (2021-11-11)
Memory T cells exhibit considerable diversity that determines their ability to be protective. Here, we examine whether changes in T cell heterogeneity contribute to the age-associated failure of immune memory. By screening for age-dependent T cell-surface markers, we identify CD4 and CD8 memory
Xiaoyuan Xie et al.
Bioscience reports, 39(7) (2019-07-04)
Adipose-derived stem cells (ADSCs) and vascular endothelial growth factor (VEGF) contribute to the healing of wound. The purpose of the present study was to investigate the role of VEGF produced by ADSCs in the protection of fibroblasts and skin of
Yao Qian et al.
Adipocyte, 10(1), 174-188 (2021-04-08)
High viability and further adipogenic differentiation of adipose-derived stem cells (ADSCs) are fundamental for engraftment and growth of the transplanted adipose tissue. It has been demonstrated that extracellular matrix (ECM) regulates cell proliferation and differentiation by interacting with ERK1/2 signalling
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Folia histochemica et cytobiologica, 55(4), 190-202 (2017-12-21)
Sirtuins are NAD dependent class III histone deacetylases. In adrenal cortex mitochondria are able to transform - via nicotinamide nucleotide transhydrogenase (NNT) - NAD into NADPH, which is required for steroidogenesis. These findings suggest that sirtuins expressed in mitochondria, Sirt3

文章

rganoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

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