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Key Documents

B2904

Sigma-Aldrich

卤代腺苷基-beta-D-半乳糖苷

reagent for selection of recombinant bacterial clones

同義詞:

5-溴-3-吲哚基β-D-吡喃半乳糖苷, 卤代腺苷基-beta-D-半乳糖苷

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About This Item

經驗公式(希爾表示法):
C14H16BrNO6
CAS號碼:
分子量::
374.18
Beilstein:
1550140
MDL號碼:
分類程式碼代碼:
12352200
PubChem物質ID:
NACRES:
NA.85

等級

for molecular biology

品質等級

無菌

non-sterile

化驗

≥98% (HPLC)

形狀

powder

技術

nucleic acid detection: suitable

溶解度

DMF: soluble

適合性

suitable for substrate for β-galactosidase

儲存溫度

−20°C

SMILES 字串

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1

InChI 密鑰

LINMATFDVHBYOS-MBJXGIAVSA-N

尋找類似的產品? 前往 產品比較指南

一般說明

Bluo-Gal是β-半乳糖苷酶的显色底物,用于确定琼脂平板上生长的细菌中是否存在克隆的DNA插入片段。Bluo-Gal设计用于具有lac +表型的重组细菌菌落的蓝白色选择中替代X-Gal。

應用

Blu-Gal适用于选择具有lac +表型的重组细菌菌落。Bluo-Gal适合作为β-半乳糖苷酶底物用于皮肤组织的电镜分析染色,网织红细胞样品和成年小鼠腔静脉的染色。
适用于鉴定lac+细菌菌落的显色底物。5-溴-4-氯-3-吲哚基 β 的替代物-D-吡喃半乳糖苷 (X-Gal),可产生较深的蓝色。

原則

当Bluo-Gal被β-半乳糖苷酶水解时,所得产物会形成蓝色沉淀。在Bluo-Gal存在的情况下,Lac +菌落的蓝色比X-Gal更深,从而能够区分Lac +和Lac-菌落。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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