跳轉至內容
Merck
全部照片(3)

Key Documents

更多文件

B9146

Sigma-Aldrich

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

≥98%

同義詞:

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, BCIG, X-Gal

登入查看組織和合約定價
全部照片(3)

About This Item

經驗公式（希爾表示法）:
C14H15BrClNO6
CAS號碼:
7240-90-6
分子量：:
408.63
Beilstein:
1402009
EC號碼:
230-640-8
MDL號碼:
MFCD00005666
分類程式碼代碼:
12352204
PubChem物質ID:
57653968
NACRES:
NA.83

品質等級

200

化驗

≥98%

形狀

powder

溶解度

DMF: methanol (1:1): soluble 50 mg/mL

適合性

suitable for identification of lac+ bacterial colonies

儲存溫度

−20°C

SMILES 字串

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI 密鑰

OPIFSICVWOWJMJ-AEOCFKNESA-N

尋找類似的產品？ 前往 產品比較指南

相關類別

應用

5-溴-4-氯-3-吲哚 β-D-半乳吡喃糖苷已被用于验证Caki-2/LacZ克隆的转导是否成功。它还可用于转化子的蓝白班筛选。
X-Gal是β-半乳糖苷酶的发色底物，可产生丰富的蓝色，与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。

重構

储备溶液（100 mg/ml）是通过将 X-Gal 溶解在二甲基甲酰胺中制备的，该溶液应避光储存在 −20 °C。无需灭菌。

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes
Bomer M, et al.
Bio-protocol, 8(1), 529-529 (2018)
HSV-tk gene therapy for human renal cell carcinoma in nude mice
Pulkkanen K, et al.
Cancer Gene Therapy, 8(7), 529-529 (2001)
Hiroyuki Kanazawa et al.
PloS one, 6(4), e19195-e19195 (2011-05-12)
Ischemia-reperfusion (I/R) injury associated with living donor liver transplantation impairs liver graft regeneration. Mesenchymal stem cells (MSCs) are potential cell therapeutic targets for liver disease. In this study, we demonstrate the impact of MSCs against hepatic I/R injury and hepatectomy.
Khanh-Van Tran et al.
Cell metabolism, 15(2), 222-229 (2012-02-14)
Adipose tissue expansion involves the enlargement of existing adipocytes, the formation of new cells from committed preadipocytes, and the coordinated development of the tissue vascular network. Here we find that murine endothelial cells (ECs) of classic white and brown fat
Enzo R Porrello et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(1), 187-192 (2012-12-19)
We recently identified a brief time period during postnatal development when the mammalian heart retains significant regenerative potential after amputation of the ventricular apex. However, one major unresolved question is whether the neonatal mouse heart can also regenerate in response
查看所有相關論文

文章

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.

條款

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務