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B9146

Sigma-Aldrich

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

≥98%, powder, suitable for identification of lac+ bacterial colonies

同義詞:

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, BCIG, X-Gal

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About This Item

經驗公式(希爾表示法):
C14H15BrClNO6
CAS號碼:
分子量::
408.63
Beilstein:
1402009
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83

product name

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, ≥98%

化驗

≥98%

形狀

powder

溶解度

DMF: methanol (1:1): soluble 50 mg/mL

適合性

suitable for identification of lac+ bacterial colonies

儲存溫度

−20°C

SMILES 字串

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI 密鑰

OPIFSICVWOWJMJ-AEOCFKNESA-N

尋找類似的產品? 前往 產品比較指南

應用

5-溴-4-氯-3-吲哚 β-D-半乳吡喃糖苷已被用于验证Caki-2/LacZ克隆的转导是否成功。它还可用于转化子的蓝白班筛选。
X-Gal是β-半乳糖苷酶的发色底物,可产生丰富的蓝色,与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。

重構

储备溶液(100 mg/ml)是通过将 X-Gal 溶解在二甲基甲酰胺中制备的,该溶液应避光储存在 −20 °C。无需灭菌。

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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文章

General protocols for growth of competent cells and their transformation (uptake of DNA).

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.

條款

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

General protocols for growth of competent cells in microbial medium.

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