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Key Documents

A9205

Sigma-Aldrich

白蛋白 溶液 来源于牛血清

30% in saline, fatty acid free, aseptically filled

同義詞:

BSA溶液

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.27

生物源

bovine

品質等級

無菌

aseptically filled

化驗

28-32% protein basis (biuret)

形狀

solution

純化經由

cold ethanol fractionation

不包含

stabilizer or preservative

來源

USA origin

濃度

28-32% protein (biuret)
30% in saline

free fatty acid content

≤0.01%

技術

ELISA: suitable
electrophoresis: suitable
western blot: suitable

雜質

≤0.01% free fatty acid

UniProt登錄號

異物活動

BT virus, none detected
VSV virus, none detected

儲存溫度

2-8°C

基因資訊

bovine ... ALB(280717)

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相關類別

一般說明

牛血清白蛋白(BSA)具有三个同源结构域,每个结构域将其细分为两个结构域(A和B)。它是一种α-螺旋球状蛋白,与人血清白蛋白具有相同的结构特征。

應用

牛血清白蛋白溶液已用于:
  • 作为Dulbecco′s改良Eagle′s培养基的补充剂用于培养HepG2人肝癌症细胞
  • 作为肝细胞复苏培养基的组分
  • 作为绵羊体外复溶胚胎的融合和活化缓冲液的组分

生化/生理作用

牛血清白蛋白(BSA)是血液中主要的蛋白,具有钙结合位点并调节金属稳态。它是细胞培养基的一种重要组成部分,可促进胚胎干细胞(hESC)的分化。由于其具有高溶解度、低成本和纯度等优点,被广泛应用于化妆品和医药工业。 BSA在酶联免疫吸附测定(ELISA)中可用作封闭剂以防止抗原和抗体与微量滴定板的非特异性结合。

準備報告

采用冷乙醇沉淀分离法进行初次分级分离

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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Astrocytes participate in both physiological and pathophysiological responses to metabolic and nutrient signals. Although most studies have focused on the astrocytic response to weight gain due to high-fat/high-carbohydrate intake, surplus intake of a balanced diet also induces excess weight gain.
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