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Key Documents

A5963

Sigma-Aldrich

抗-c-Myc

clone 9E10, purified from hybridoma cell culture

同義詞:

Anti-c-Myc, Monoclonal Anti-c-Myc−Alkaline Phosphatase antibody produced in mouse

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

共軛

alkaline phosphatase conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

9E10, monoclonal

形狀

buffered aqueous glycerol solution

物種活性

human

技術

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: 1:100 using a myc-tagged fusion protein.

同型

IgG1

UniProt登錄號

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... MYC(4609)

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一般說明

Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. The c-myc gene encodes for a protein of 62kDa called p62c-myc is associated mainly with cell nuclei.

免疫原

人p62c-Myc蛋白的合成肽。

應用

Anti-c-Myc-Alkaline Phosphatase antibody has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemical labeling
  • immunostaining
  • western blotting

Monoclonal Anti-c-Myc-Alkaline Phosphatase antibody can be used for western blot assays at 1:100, using myc-tagged fusion proteins. The antibody can also be used for immunohistochemical applications.

生化/生理作用

The c-myc oncogene product(p62c-myc) exerts normal and oncogenic cellular functions. Elevated level of c-myc protein in malignant tissues when compared with normal tissue, but with the unexpected finding of a cytoplasmic accumulation of the protein in these tumors.

外觀

Solution in 0.05 M Tris buffer, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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存取文件庫

Structural and functional insights into the lipopolysaccharide ABC transporter LptB 2 FG
Dong H, et al.
Nature Communications, 8(1), 222-222 (2017)
Ubiquitin ligase SYVN1/HRD1 facilitates degradation of the SERPINA1 Z variant alpha-1-antitrypsin Z variant via SQSTM1/p62-dependent selective autophagy
Feng,L, et al.
Autophagy, 13(4), 686-702 (2017)
Novel ganglioside antigen identified by B cells in human medullary breast carcinomas: the proof of principle concerning the tumor-infiltrating B lymphocytes
Kotlan B, et al.
Journal of Immunology, 175(4), 2278-2285 (2005)
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Evan G I, et al.
Molecular and Cellular Biology, 5(12), 3610-3616 (1985)
Beatrix Kotlan et al.
Journal of immunology (Baltimore, Md. : 1950), 175(4), 2278-2285 (2005-08-06)
The potential tumor-recognizing capacity of B cells infiltrating human breast carcinoma is an important aspect of breast cancer biology. As an experimental system, we used human medullary breast carcinoma because of its heavy B lymphocytic infiltration paralleled to a relatively

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