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Key Documents

A3316

Sigma-Aldrich

抗-人IgG(Fc特异性)−琼脂糖 山羊抗

affinity isolated antibody, PBS suspension

同義詞:

Goat anti-human IgG

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

共軛

agarose conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

PBS suspension

物種活性

human

應無反應活性

rat, mouse

技術

Ouchterlony double diffusion: suitable

容量

2 mg/mL, resin binding capacity (human IgG)

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

人IgG是糖蛋白抗体,含有两条相等的轻链和一对相同的重链。 IgG具有四种不同亚型,范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。
当针对纯化的人IgA、IgG、IgM、Fc以及κ和λ轻链进行测试时,山羊抗人IgG(Fc特异性)-琼脂糖抗体对人IgG的Fc片段具有特异性。在琼脂糖珠偶联之前,通过Ouchterlony双扩散(ODD)未观察到与小鼠或大鼠IgG的反应性。

免疫原

人IgG的Fc片段。

應用

使用与琼脂糖珠交联的山羊抗人IgG(Fc特异性)进行免疫沉淀测定。将珠子在1 ml的5%FBS/DMEM中于4℃下稀释2小时。
山羊产生的抗人IgG(Fc特异性)-琼脂糖抗体也已用于从转染的COS细胞上清液中纯化甘丙肽1(Gala1),3Gal取代的P-选择蛋白糖蛋白配体1(PSGL-1)/hIgG1。
羊抗人IgG(Fc特异性)-琼脂糖抗体适用于Ouchterlony双扩散。该抗体还可用于亲和纯化测定。

其他說明

小鼠和大鼠 IgG 吸附的抗体

外觀

0.01 M 磷酸盐缓冲盐水(pH 7.4)混悬液,含 15 mM 叠氮化钠

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


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Rebecca J Brown et al.
The Journal of clinical endocrinology and metabolism, 102(6), 1789-1791 (2016-12-03)
Hyperinsulinemia can lead to pathologic ovarian growth and androgen production. A 29-year-old woman developed an autoantibody to the insulin receptor (type B insulin resistance), causing extreme insulin resistance and hyperinsulinemia. Testosterone levels were elevated to the adult male range. Treatment
J L Xu et al.
Immunity, 13(1), 37-45 (2000-08-10)
All rearranging antigen receptor genes have one or two highly diverse complementarity determining regions (CDRs) among the six that typically form the ligand binding surface. We report here that, in the case of antibodies, diversity at one of these regions
J Liu et al.
Transplantation, 63(11), 1673-1682 (1997-06-15)
The hyperacute rejection caused by preformed natural antibodies in the recipient species reacting with donor species endothelial antigens is one of the major obstacles preventing routine use of clinical xenotransplantation. Based on the known structure and biosynthetic pathway of the
S Sumitran et al.
Experimental neurology, 159(2), 347-361 (1999-10-03)
Transplantation of porcine embryonic brain cells, including dopaminergic neurons, from ventral mesencephalon (VM) is considered a potential treatment for patients with Parkinson's disease. In the present study, we characterized the distribution among VM cells of the major porcine endothelial xenoantigen
Jining Liu et al.
Xenotransplantation, 10(2), 149-163 (2003-02-18)
Hyperacute organ xenograft rejection can be prevented by removing anti-pig antibodies by extracorporeal absorption prior to transplantation. A novel recombinant absorber of anti-pig antibodies was developed by fusing the cDNA encoding the extracellular part of a mucin-type protein, P-selectin glycoprotein

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