A2582

Anti-Thioredoxin–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

• 同義詞: Anti-Thioredoxin
• 分類程式碼代碼: 12352203
• NACRES: NA.46
• 共軛: agarose conjugate
• application: IP; affinity chromatography
• 無性繁殖: polyclonal
• 物種活性: E. coli
• citations: 3
• 技術: affinity chromatography: suitable; immunoprecipitation (IP): suitable

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: agarose conjugate
• 抗體表格: IgG fraction of antiserum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: PBS suspension
• 分子量: antigen 11.7 kDa
• 物種活性: E. coli
• 分析物化學類別: proteins (thioredoxin)
• 技術: affinity chromatography: suitable; immunoprecipitation (IP): suitable
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

Thioredoxin is a small electron transport protein. The thioredoxin system consists of thioredoxin, thioredoxin-reductase and NADPH. Thioredoxin obtained from E. coli is made up of a single polypeptide chain of 108 amino acids. It has a molecular weight of 11,700. The protein contains no prosthetic group or bound metals.

特異性

Specific for natural E. coli and recombinant thioredoxin. It may be used to identify and purify the expression of thioredoxin fusion proteins.

免疫原

recombinant E. coli thioredoxin.

應用

Anti-Thioredoxin−Agarose antibody produced in rabbit has been used for immunoaffinity purification and immunoprecipitation of thioredoxin from bacterial lysates.

Immunofluorescence was carried out on the cerivcal cancer cell lines SiHa, CaSki, and HeLa using an antibody against the redox proteinThioredoxin.

生化／生理作用

Thioredoxin is a 11.7kDa electron transport protein that functions as a hydrogen donor during the enzymatic reduction of ribonucleotides to yield deoxyribonucleotides. The thioredoxin system is also involved in the enzymatic reductions of methionine sulfoxide and sulfate. The gene encoding E. coli thioredoxin has been cloned into several plasmids and serves as a fusion partner for the expression of other cloned genes. Hence, the system is useful for the production of soluble fusion proteins in the E. coli cytoplasm. Furthermore, in several instances, these fusion proteins fold correctly and thus display full biological activity.

外觀

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

準備報告

Prepared using antibody coupled to cyanogen bromide activated agarose at 5 mg antibody per mL resin volume.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.