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Merck
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重要文件

A2289

Sigma-Aldrich

白蛋白,四甲基异硫氰酸罗丹明 牛

lyophilized powder

同義詞:

TRITC-albumin

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.25

生物源

bovine

品質等級

化驗

≥90% (Lowry)

形狀

lyophilized powder

標籤範圍

≥0.5 mol TRITC per mol albumin

技術

cell culture | mammalian: suitable

雜質

salt, free

UniProt登錄號

儲存溫度

2-8°C

基因資訊

bovine ... ALB(280717)

一般說明

Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).

應用

Albumin, Tetramethylrhodamine isothiocyanate has been used:
  • in photoinduced release assay of polyethylene beads
  • for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
  • in fluorescent correlation spectroscopy

Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.

準備報告

Prepared from crystallized and lyophilized albumin (A4378).

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Photoresponsive Aqueous Dissolution of Poly (N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
Sumaru K, et al.
Biomacromolecules, 19(7), 2913?2922-2913?2922 (2018)
Tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection by incorporation of red-fluorescing tetramethylrhodamine isothiocyanate-bovine serum albumin into these nanoparticles
Sokolova V, et al.
Journal of Biological Inorganic Chemistry, 12(2), 174-179 (2007)
Fluorescent Biorecognition of Gold Nanoparticle-IgG Conjugates Self-Assembled on E-Beam Patterns
Powell T and Yoon JY
Biotechnology Progress, 22(1), 106-110 (2006)
Correct diffusion coefficients of proteins in fluorescence correlation spectroscopy. Application to tubulin oligomers induced by Mg2+ and Paclitaxel
Krouglova T, et al.
Biophysical Journal, 87(4), 2635-2646 (2004)
Evaluation of fluorescent plasma markers for in vivo microscopy of the microcirculation
Reeves KJ, et al.
Journal of Vascular Research, 49(2), 132-143 (2012)

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