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G2500

Sigma-Aldrich

明胶 来源于猪皮肤

gel strength 300, Type A

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.61

生物源

Porcine skin

品質等級

種類

Type A

化驗

70-90% (biuret)

形狀

powder

技術

cell culture | mammalian: suitable

凝膠強度

~300 g Bloom

溶解度

H2O: soluble 50 mg/mL

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一般說明

明胶是一种亲水胶体。商业生产的明胶来自于牛骨、猪骨和兽皮。作为胶原部分水解产物,主要成分为蛋白质,其他为矿物质和水。

應用

明胶(来源于猪皮)已用于:
  • 包被培养板玻璃底部,以便培养A75细胞和进行明胶降解分析
  • 包被玻片,以进行脑切片组织学研究
  • 合成甲基丙烯酸酯化明胶(GelMA)预聚物,用于细胞封装和3D打印水凝胶
  • 制备巯基化明胶(GelSH)水凝胶,用于3D生物打印(3D projection bioprinting)

明胶(来源于猪皮)建议以1-5 μg/cm2或0.5-50 μg/ml用作细胞培养基底。最佳的浓度的确取决于细胞类型以及应用和研究目标。

生化/生理作用

明胶聚合物可作为胶原的生物材料替代物,用于细胞和组织培养。作为精挑细选的胶凝剂,可用作增稠剂和食品配料。明胶还可用作发泡剂和黏合剂,以及用于医药行业。因生物可降解性和生物相容性,适合生物医药应用,尤其适合作为心血管组织再生的生物材料。

成分

明胶存在于胶原蛋白中,是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

注意

干燥的明胶,当在室温下储存于密闭容器中时,将可保持多年不变。 当在空气存在时在100℃下加热时,它会溶胀变软,并分解成碳质物质并伴有吡啶碱和氨的释放。

準備報告

该产品源自于猪皮。明胶可溶于热水而非冷水。 它几乎不溶于大多数有机溶剂,如醇、氯仿、二硫化碳、四氯化碳、乙醚、苯、丙酮和油。 由Bloom凝胶计所测定的Bloom指数,可表示由已知浓度溶液而形成的凝胶的强度。 Bloom指数与平均分子量成正比。 猪皮明胶的Bloom值在90至300 g之间。本品的胶冻强度达300,可溶于水(50 mg/mL)。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Gabriele Pitingolo et al.
Micromachines, 10(4) (2019-04-24)
Traditional two-dimensional (2D) cell culture models are limited in their ability to reproduce human structures and functions. On the contrary, three-dimensional (3D) microtissues have the potential to permit the development of new cell-based assays as advanced in vitro models to
Sami Zaqout et al.
Frontiers in neuroanatomy, 10, 38-38 (2016-04-12)
Golgi staining remains a key method to study neuronal morphology in vivo. Since most protocols delineating modifications of the original staining method lack details on critical steps, establishing this method in a laboratory can be time-consuming and frustrating. Here, we
Nicola Contessi Negrini et al.
Acta biomaterialia, 87, 61-75 (2019-01-18)
When adipose tissue (AT) is impaired by trauma or disease, AT engineering could provide a shelf-ready structural and functional restoration as alternative to current clinical treatments, which mainly aim at aesthetic replacement. Yet, the lack of an efficient vascular network
R F Vogt et al.
Journal of immunological methods, 101(1), 43-50 (1987-07-16)
We tested instantized dry milk, casein, gelatins from pig and fish skin, serum albumin and several other proteins for their abilities to block non-specific binding (NSB) of a peroxidase-conjugated immunoglobulin to polystyrene microtiter plate wells. Each blocking protein was tested
J P Gobetti et al.
Journal of the American Dental Association (1939), 113(2), 291-292 (1986-08-01)
The study showed that washing a gloved hand removed significant amounts of bacteria. If the proper soap or scrub is used, the gloved hand will be free of bacteria. It is suggested that all dental personnel wear gloves to protect

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