A211150

DMT-2′O-Methyl-rA(bz) 磷酰胺

configured for (ÄKTA^® and OligoPilot^®), configured for ABI

• 同義詞: DMT-2′-O-甲基-rA(bz)酰胺, N-苯甲酰-5′-O- [双（4-甲氧苯基）苯甲基]-2′-O-甲基-腺苷，3′-[2-氰乙基 N,N-双（1-甲基乙基）亚磷酰胺]
• 經驗公式（希爾表示法）: C₄₈H₅₄N₇O₈P
• CAS號碼: 110782-31-5
• 分子量：: 887.96
• MDL號碼: MFCD00792622
• 分類程式碼代碼: 41116105
• PubChem物質ID: 329770744
• NACRES: NA.51

屬性

• 生物源: non-animal source (no BSE/TSE risk)
• 品質等級: 300
• 產品線: Proligo Reagents
• 化驗: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• 形狀: powder
• 技術: oligo synthesis: suitable
• 雜質: ≤0.1% single unspecified Impurity (reversed phase HPLC); ≤0.3% mA2 (reversed phase HPLC, Hydrolysate); ≤0.3% mA3 (reversed phase HPLC, DMT-rA(bz)me); ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤1.0% mA1 (reversed phase HPLC, DMT-rA(bz)me-DMT); ≤3% residual Solvent content
• 顏色: white to off-white
• &lambda ；: conforms (UV/VIS Identity)
• 適合性: conforms to structure for H-NMR; conforms to structure for LC-MS
• 相容性: configured for (ÄKTA^® and OligoPilot^®); configured for ABI
• 核苷譜: base: adenosine; base protecting group: benzoyl; 2' protecting group: methyl; 5' protecting group: DMT; deprotection: standard
• 儲存溫度: 2-8°C
• SMILES 字串: CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1n5cnc6c(NC(=O)c7ccccc7)ncnc56
• InChI: 1S/C48H54N7O8P/c1-32(2)55(33(3)4)64(61-28-14-27-49)63-42-40(62-47(43(42)59-7)54-31-52-41-44(50-30-51-45(41)54)53-46(56)34-15-10-8-11-16-34)29-60-48(35-17-12-9-13-18-35,36-19-23-38(57-5)24-20-36)37-21-25-39(58-6)26-22-37/h8-13,15-26,30-33,40,42-43,47H,14,28-29H2,1-7H3,(H,50,51,53,56)/t40-,42-,43-,47-,64?/m1/s1
• InChI 密鑰: AZCGOTUYEPXHMJ-PSVHYZMASA-N

描述

一般說明

DMT-2′O-Methyl-rA(bz) Phosphoramidite is a 2′O-Methyl RNA monomer that is compatible with fast deprotection schemes that are based on the application of aliphatic amines, such as methylamine. Modern phosphoramidite-mediated synthesis has enabled routine high-yielding preparations of 2′O-Methyl RNA oligonucleotides. High-quality RNA phosphoramidites are key to low failure rates, the high biological activity of synthesis products, and cost-effectiveness. 2′O-Methyl RNA is a nucleic acid analog that is characterized by the exceptional hybridization properties that it imparts with complementary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids.

應用

2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rA(bz) Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of:

• Diagnostic probes
• Aptamer and ribozyme development
• Mixed 2′O-Methyl-RNA/DNA antisense molecules (a)

法律資訊

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable