U211150

DMT-2′O-Methyl-rU Phosphoramidite

configured for ÄKTA^® and OligoPilot^®

• 同義詞: DMT-2′O-甲基-rU酰胺
• 經驗公式（希爾表示法）: C₄₀H₄₉N₄O₉P
• CAS號碼: 110764-79-9
• 分子量：: 760.81
• MDL號碼: MFCD00792626
• 分類程式碼代碼: 12352200
• PubChem物質ID: 329827609
• NACRES: NA.51

屬性

• 生物源: non-animal source (no BSE/TSE risk)
• 品質等級: 300
• 產品線: Proligo Reagents
• 化驗: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• 形狀: powder
• 技術: oligo synthesis: suitable
• 雜質: ≤0.1% single unspecified Impurity (reversed phase HPLC); ≤0.3% mU2 (reversed phase HPLC, Hydrolysate); ≤0.3% mU3 (reversed phase HPLC, DMT-rme); ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT); ≤3% residual Solvent content
• 顏色: white to off-white
• &lambda ；: conforms (UV/VIS Identity)
• 適合性: conforms to structure for H-NMR; conforms to structure for LC-MS
• 相容性: configured for ÄKTA^® and OligoPilot^®
• 核苷譜: base: uridine; base protecting group: none; 2' protecting group: methyl; 5' protecting group: DMT; deprotection: fast/standard
• 儲存溫度: 2-8°C
• SMILES 字串: CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1N5C=CC(=O)NC5=O
• InChI: 1S/C40H49N4O9P/c1-27(2)44(28(3)4)54(51-25-11-23-41)53-36-34(52-38(37(36)49-7)43-24-22-35(45)42-39(43)46)26-50-40(29-12-9-8-10-13-29,30-14-18-32(47-5)19-15-30)31-16-20-33(48-6)21-17-31/h8-10,12-22,24,27-28,34,36-38H,11,25-26H2,1-7H3,(H,42,45,46)/t34-,36-,37-,38-,54?/m1/s1
• InChI 密鑰: UVUOJOLPNDCIHL-XKZJCBTISA-N

描述

一般說明

DMT-2′O-Methyl-rU Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites.

應用

2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rU Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.

特點和優勢

Its key features include:

• High yield of crude oligonucleotides
• Compatible with DNA synthesis
• Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides
• Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA (concentrated ammonia/ 40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C
• Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation
• Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers)
• 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry

其他說明

The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of:

• Diagnostic probes
• Aptamer and ribozyme development
• Mixed 2′O-Methyl-RNA/DNA antisense molecules

法律資訊

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable