A1812

单克隆抗-FITC

clone FL-D6, purified immunoglobulin, buffered aqueous solution

• 同義詞: Monoclonal Anti-FITC
• 分類程式碼代碼: 12352203
• NACRES: NA.46

屬性

• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in mouse cell line
• 共軛: alkaline phosphatase conjugate
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: FL-D6, monoclonal
• 形狀: buffered aqueous solution
• 技術: dot blot: 1:80,000 using human IgG/FITC monoclonal anti-human IgG; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using FITC conjugate; indirect ELISA: 1:30,000
• 同型: IgG1
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten. Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

特異性

该抗体可与游离的和结合的异硫氰酸荧光素（FITC）反应。

免疫原

FITC-BSA偶联物

應用

Alkaline phosphatase-conjugated mouse anti-FITC antibody was used for immunohistochemistry on mouse spleens. Tissue sections were fixed in acetone, blocked with PBS/5% goat serum/ 0.1% Tween and incubated with a FITC-conjugated primary antibody prior to the addition of the AP-conjugated anti-FITC antibody.

Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent such as alkaline phosphatase or horseradish peroxidase. Mouse monoclonal clone FL-D6 anti-FITC−Alkaline Phosphatase conjugate antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It can be used in ELISA and immunofluorescent techniques.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

外觀

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 50% glycerol, 1 mM MgCl₂ and 15 mM sodium azide

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)