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Key Documents

93352

Sigma-Aldrich

Trizma ®

≥99.0% (T), crystalline, aminopeptidase substrate, BioChemika

同義詞:

2-氨基-2-(羟甲基)-1,3-丙二醇, THAM, Tris 碱, 三羟甲基氨基甲烷, 氨基丁三醇

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About This Item

線性公式:
NH2C(CH2OH)3
CAS號碼:
分子量::
121.14
Beilstein:
741883
EC號碼:
MDL號碼:
分類程式碼代碼:
12352104
PubChem物質ID:
NACRES:
NA.25

product name

Trizma ® 碱, ≥99.0% (T)

描述

aminopeptidase substrate

品質等級

產品線

BioChemika

化驗

≥99.0% (T)

形狀

crystalline

損耗

≤1% loss on drying, 110 °C

pH值

10.5-12.0(4 m in water, 25 °C)

有用的pH值範圍

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

溶解度

H2O: 1 M at 20 °C, clear, colorless

負離子痕跡

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

正離子痕跡

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES 字串

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI 密鑰

LENZDBCJOHFCAS-UHFFFAOYSA-N

尋找類似的產品? 前往 產品比較指南

一般說明

Tris 是生物化学和分子生物学中已确立的基础标准品和缓冲液。可单独作为缓冲液使用,也可作为混合缓冲液配方的组分,如 Tris-EDTA (TE) 缓冲液、Tris-acetate-EDTA (TAE) 缓冲液、Tris-borate-EDTA (TBE) 缓冲液等。它纯净,基本稳定,相对不吸湿,具有很高的当量。

應用

Trizma ® 碱用作以下研究的缓冲液:
  • 电泳转移法特异性鉴定淀粉脱支酶 α 的同工酶-淀粉酶和 9-淀粉酶。
  • 聚丙烯酰胺凝胶中脂蛋白的电泳分离。
  • 制备 pH 值为 8 的 TRIS 缓冲液。
可组成 DN 缓冲液,用于组织切片的 DNA 缺口末端标记。

其他說明

所有缓冲液的 pH 值都取决于温度和浓度。Tris 缓冲液,温度每降低 1°C,pH 值增加约 0.03 个单位,每稀释十倍 pH 值降低0.03-0.05 个单位。
对于精确的应用,使用经过精心校准的 pH 计和玻璃/甘汞组合电极。

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Cyril Lafon et al.
Ultrasound in medicine & biology, 31(10), 1383-1389 (2005-10-15)
An optically transparent phantom was developed for use in high-intensity focused ultrasound (US), or HIFU, dosimetry studies. The phantom is composed of polyacrylamide hydrogel, embedded with bovine serum albumin (BSA) that becomes optically opaque when denatured. Acoustic and optical properties
Electrophoretic separation of serum lipoproteins in polyacrylamide gel.
C S Frings et al.
Clinical chemistry, 17(2), 111-114 (1971-02-01)
J M Isner et al.
Circulation, 91(11), 2703-2711 (1995-06-01)
Apoptosis has been recognized in normal, including rapidly proliferating, cell populations and is inferred to be potentially responsible for the maintenance of stable cell numbers in tissues with various degrees of proliferative activity. Previous studies performed in rats indicated that
G Kakefuda et al.
Plant physiology, 75(1), 278-280 (1984-05-01)
An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, alpha-amylase, and beta-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels.
Marcel Kuiper et al.
Biotechnology progress, 35(4), e2821-e2821 (2019-04-16)
Perfusion is a cell culture mode that is gaining popularity for the manufacture of monoclonal antibodies and their derivatives. The cell culture media supporting perfusion culture need to support higher cell densities than those used in fed-batch culture. Therefore, when

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