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重要文件

93289

Sigma-Aldrich

TRI试剂®

同義詞:

TRI试剂® RNA 分离试剂

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About This Item

MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.52

形狀

liquid

用途

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue

雜質

phenol

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一般說明

TRI reagent®也称为TRIzol,是苯酚和异硫氰酸胍的单相溶液。它可能同时溶解生物材料并使蛋白质变性。这一产品广泛用于RNA脱蛋白。由Chomczynski开发的这种改进版的一步总RNA分离试剂可以快速、经济、有效地分离来自不同生物样品的总RNA。该产品是纯净的(A260/A280 ~1.8)并且可以直接用于Northern印迹。还可同时分离DNA、RNA和蛋白质。1 mL足以用于提取100 mg组织或107个细胞。

應用

TRI Reagent®已用于从各类细胞或组织中分离RNA。这一产品还可以用于处理组织、单层培养细胞或细胞团块。
TRI试剂是对由Chomczynski开发的一步法总RNA分离试剂进行改良的一种版本。基于该试剂的RNA分离方法已被广泛用于和验证用于RNA应用。它是对来自人、动物、植物、酵母、细菌和病毒来源样本进行总RNA或RNA、DNA和蛋白同时分离的一种理想的快速、经济和高效的方法。

特點和優勢

  • 可轻松扩展的RNA分离
  • 可适用于多种来源:人、植物、酵母、细菌或病毒
  • 相对于传统的硫氰酸胍/氯化铯具有更高的产量

法律資訊

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

標靶器官

Nervous system,Kidney,Liver,Skin

安全危害

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 2

閃點(°F)

174.2 °F - closed cup

閃點(°C)

79 °C - closed cup

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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文章

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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