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重要文件

92846

Sigma-Aldrich

5-羧基荧光素 N -琥珀酰亚胺酯

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

經驗公式(希爾表示法):
C25H15NO9
CAS號碼:
分子量::
473.39
Beilstein:
7865828
MDL號碼:
分類程式碼代碼:
12352108
PubChem物質ID:
NACRES:
NA.32

產品線

BioReagent

品質等級

化驗

≥90% (HPLC)

形狀

powder

螢光

λex 492 nm; λem 520 nm in 0.1 M Tris pH 8.0

適合性

suitable for fluorescence

儲存溫度

−20°C

SMILES 字串

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O

InChI

1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)

InChI 密鑰

TWOKNYRPKCLMAR-UHFFFAOYSA-N

包裝

无底玻璃瓶。内含物装在插入的融合锥内。

其他說明

荧光素衍生物在与生物聚合物偶联时产生稳定的衍生物。核苷酸的荧光探针 ;毛细管电泳荧光衍生试剂

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Bamaprasad Dutta et al.
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Atherosclerosis arises from leukocyte infiltration and thickening of the artery walls and constitutes a major component of vascular disease pathology, but the molecular events underpinning this process are not fully understood. Proteins containing an Asn-Gly-Arg (NGR) motif readily undergo deamidation
Lau, S.K., et al.
Journal of Chromatography A, 809, 203-203 (1998)
G L Igloi
Analytical biochemistry, 233(1), 124-129 (1996-01-01)
A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluorescein. The product of
L G Lee et al.
Nucleic acids research, 20(10), 2471-2483 (1992-05-25)
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments
Brandon L Blakely et al.
Nature methods, 11(12), 1229-1232 (2014-10-13)
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to

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Fluorescent Labeling of Peptides

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