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重要文件

83330

Sigma-Aldrich

丙酮酸激酶 来源于兔肌肉

crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)

同義詞:

ATP:丙酮酸盐 2-O-磷酸转移酶, PK

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

形狀

suspension

品質

crystalline suspension in 3.8 M ammonium sulfate solution

比活性

~350 U/mg protein (10-40 mg/ml)

分子量

Mr ~230000

pH值

~6

異物活動

lactate dehydrogenase ≤0.01%

儲存溫度

2-8°C

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生化/生理作用

分子量:237 kDa,以分子量为 57 kDa 的四个等亚基的四聚体形式存在。
等电点:7.6
最佳 pH:~7.5
最佳温度:25°C
εA 280= 0.541 mg (p)/ml,1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中,报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性,但可利用其他三磷酸二核苷酸作为底物替代 ATP,包括 GTP、ITP、dATP、UTP 和 CTP。
Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.

單位定義

1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Nicolas Galazis et al.
European journal of endocrinology, 168(2), R33-R43 (2012-10-25)
Women with polycystic ovary syndrome (PCOS) are at increased risk of developing insulin resistance and type 2 diabetes mellitus (T2DM). In this study, we attempted to list the proteomic biomarkers of PCOS and T2DM that have been published in the
William J Cook et al.
PloS one, 7(10), e46875-e46875 (2012-10-12)
Pyruvate kinase plays a critical role in cellular metabolism of glucose by serving as a major regulator of glycolysis. This tetrameric enzyme is allosterically regulated by different effector molecules, mainly phosphosugars. In response to binding of effector molecules and substrates
Daniel I Benjamin et al.
Cell metabolism, 16(5), 565-577 (2012-10-16)
Cancer cells possess fundamentally altered metabolism that provides a foundation to support tumorigenicity and malignancy. Our understanding of the biochemical underpinnings of cancer has benefited from the integrated utilization of large-scale profiling platforms (e.g., genomics, proteomics, and metabolomics), which, together
Mayumi Tamada et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 18(20), 5554-5561 (2012-10-17)
The M2 splice isoform of pyruvate kinase (PKM2), an enzyme that catalyzes the later step of glycolysis, is a key regulator of aerobic glycolysis (known as the Warburg effect) in cancer cells. Expression and low enzymatic activity of PKM2 confer
Jidong Cheng et al.
Genes to cells : devoted to molecular & cellular mechanisms, 17(11), 913-922 (2012-10-20)
AMP deaminase (AMPD) catalyzes AMP to IMP and plays an important role in energy charge and nucleotide metabolism. Human AMPD3 deficiency is a type of erythrocyte-specific enzyme deficiency found in individuals without clinical symptoms, although an increased level of ATP

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