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56304

Sigma-Aldrich

Atto 565-Streptavidin

BioReagent, suitable for fluorescence

同義詞:

Streptavidin-Atto 565

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About This Item

MDL號碼:
分類程式碼代碼:
12352108
NACRES:
NA.32

產品線

BioReagent

品質等級

製造商/商標名

ATTO-TEC GmbH

螢光

λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0

適合性

corresponds for gel electrophoresis
suitable for fluorescence

儲存溫度

−20°C

應用

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). It does nearly not show cis trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Streptavidin conjugation of Atto 565 does not significantly change the labels spectral data regarding excitation and emission maxima. Atto 565-Streptavidin is used to label biotin conjugated molecules.

分析報告

free of unconjugated dye

法律資訊

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Lukasz Krzemiński et al.
The journal of physical chemistry. B, 115(43), 12607-12614 (2011-09-24)
Recently, studies have been reported in which fluorescently labeled redox proteins have been studied with a combination of spectroscopy and electrochemistry. In order to understand the effect of the dye on the protein-electrode interaction, voltammetry and surface analysis have been
Judith E Berlier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(12), 1699-1712 (2003-11-19)
Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were
PALM and STORM. Unlocking live-cell super-resolution.
Henriques, R.; Griffiths, C.; Hesper Rego, E.; Mhlanga, Musa M.
Biopolymers, 95(5), 322-331 (2011)
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde, S.; et al.
Nature Protocols, 6(7), 991-1009 (2011)

文章

Fluorescent Labeling of Peptides

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