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52412

Sigma-Aldrich

R -藻红蛋白

BioReagent, passes test for gel electrophoresis

同義詞:

R-PE, 藻胆蛋白

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

產品線

BioReagent

品質等級

濃度

≥10.0 mg/mL (UV)

技術

electrophoresis: suitable

螢光

λex 542 nm; λem 575 nm in 0.1 M phosphate pH 7.2

適合性

passes test for gel electrophoresis

儲存溫度

2-8°C

一般說明

R-藻红蛋白或R-PE是一种从大型藻类中获得的藻胆蛋白类荧光探针。 R-藻红蛋白(R-PE)是一种可用作 红藻 (红藻门)光合辅助色素的蛋白质。 它是一种240 kDa的寡聚蛋白,具有6个α(约20 kDa)、6个β(约20 kDa)和1个γ(约30 kDa)亚单位。 藻胆蛋白是一种具有高荧光特性的水溶性光捕获蛋白。

應用

藻红蛋白(R-PE),在实验室中可作为蓝藻存在和各种免疫荧光应用的基于荧光的指标。在各种电泳过程中,R-PE是一种有用的荧光探针。 R-藻红蛋白(R-PE)可检测土壤和地下水样品中的汞离子。 藻红蛋白已广泛应用于食品、化妆品、免疫诊断、分析试剂等领域。

分析報告

本品混悬于150 mM磷酸钠、60%硫酸铵、1 mM EDTA、1 mM 叠氮化钠(pH 7.0)中,偶联前必须用偶联缓冲液或PBS透析。A566/A280 >4.6、A620/A566 <0.03、A566/A498 <1.5

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


分析證明 (COA)

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Xuefei Sun et al.
Journal of chromatography. A, 1216(1), 159-164 (2008-12-17)
Electric field gradient focusing (EFGF) is a technique used to simultaneously separate and concentrate biomacromolecules, such as proteins, based on the opposing forces of an electric field gradient and a hydrodynamic flow. Recently, we reported EFGF devices fabricated completely from
Joerg Martini et al.
Lab on a chip, 12(23), 5057-5062 (2012-10-10)
We describe an optical detection technique that delivers high signal-to-noise discrimination to enable a multi-parameter flow cytometer that combines high performance, robustness, compactness and low cost. The enabling technique is termed "spatially modulated detection" and generates a time-dependent signal as
Kun Tang et al.
Biochimica et biophysica acta, 1817(7), 1030-1036 (2012-04-03)
Phycobilisomes, the light-harvesting antennas in cyanobacteria and red algae, consist of an allophycocyanin core that is attached to the membrane via a core-membrane linker, and rods comprised of phycocyanin and often also phycoerythrin or phycoerythrocyanin. Phycobiliproteins show excellent energy transfer
Radek Sachl et al.
Biophysical journal, 101(11), L60-L62 (2012-01-21)
Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization of such domains. In this letter
David Rizzo et al.
Cytometry. Part B, Clinical cytometry, 84(2), 125-132 (2012-12-12)
Flow cytometry is the reference technique for assessing ZAP70 expression, a marker of poor prognosis in CLL. One of the most common methods is to assess ZAP70 levels in CLL cells by calculating the ratio between ZAP70 mean fluorescence intensities

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