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重要文件

50185

Sigma-Aldrich

抗-小鼠-IgG - Atto 647N 山羊抗

contains 50% glycerol as stabilizer

同義詞:

Atto 647N-抗-小鼠 IgG 山羊抗

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

共軛

Atto 647N conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

包含

50% glycerol as stabilizer

物種活性

mouse

濃度

≥0.8 mg/mL IgG

技術

immunofluorescence: 5 μg/mL

螢光

λex 647 nm; λem 665 nm in PBS

適合性

in accordance for fluorescence

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

免疫球蛋白G(IgG)是一种糖蛋白抗体,其可调节免疫反应,如吞噬作用,也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型,即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。Atto 647N山羊抗小鼠IgG与小鼠IgG结合。

免疫原

小鼠 IgG

應用

Atto荧光标记专门用于单分子检测等高灵敏度应用。 Atto标记的结构具有刚性,构型不会产生任何顺反异构变化。 因此这种标记偶联后的强度表现卓越,光谱转变却又极低。Atto 647N 山羊抗兔 IgG 已作为二抗(浓度为 5μg/ml),用于2% 甲醛固定细胞的免疫荧光。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析(1 篇论文)

外觀

Atto 647 山羊抗小鼠 IgG(全分子)以 1 mg/ml 溶液的形式提供,溶剂为 0.1M 磷酸钠,0.1M NaCl,pH 7.5,含有 5 mM 叠氮化钠作为防腐剂。

分析報告

未缀合染料的总荧光 ≤5%

法律資訊

本产品仅供研究使用。如果要商业化,请联系知识产权持有者(ATTO-TEC GmbH,德国)获取许可。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Beatriz Marcos-Ramiro et al.
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Endothelial barrier dysfunction underlies chronic inflammatory diseases. In searching for new proteins essential to the human endothelial inflammatory response, we have found that the endosomal GTPase RhoB is up-regulated in response to inflammatory cytokines and expressed in the endothelium of
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Tissue osmolarity varies among different organs and can be considerably increased under pathologic conditions. Hyperosmolarity has been associated with altered stimulatory properties of immune cells, especially macrophages and dendritic cells. We have recently reported that dendritic cells upon exposure to
H Singh et al.
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Large conductance voltage and calcium-activated potassium (MaxiK) channels are activated by membrane depolarization and elevated cytosolic Ca(2+). In the brain, they localize to neurons and astrocytes, where they play roles such as resetting the membrane potential during an action potential
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Traditional approaches for finding well-performing parameterizations of complex imaging systems, such as super-resolution microscopes rely on an extensive exploration phase over the illumination and acquisition settings, prior to the imaging task. This strategy suffers from several issues: it requires a
Jasmin Mertins et al.
eLife, 10 (2021-11-16)
SNARE proteins have been described as the effectors of fusion events in the secretory pathway more than two decades ago. The strong interactions between SNARE domains are clearly important in membrane fusion, but it is unclear whether they are involved

文章

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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