共軛
Atto 647N conjugate
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
liquid
包含
50% glycerol as stabilizer
物種活性
rabbit
濃度
1 mg/mL IgG
技術
immunofluorescence: suitable (5μg/ml)
螢光
λex 647 nm; λem 665 nm in PBS
適合性
in accordance for fluorescence
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
一般說明
IgG 是糖蛋白抗体，可调节几种免疫应答。针对靶蛋白的兔 IgG 常被用作各种研究应用的一抗。因此，与可检测底物偶联的二级抗兔 IgG 是分析靶蛋白的有用工具。已知山羊抗兔 IgG 与兔 IgG 相关。
免疫原
兔 IgG
應用
Atto荧光标记专门用于单分子检测等高灵敏度应用。 Atto标记的结构具有刚性，构型不会产生任何顺反异构变化。 因此这种标记偶联后的强度表现卓越，光谱转变却又极低。Atto 647N 山羊抗兔 IgG 已作为二抗（浓度为 5μg/ml），用于2% 甲醛固定细胞的免疫荧光。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫荧光分析（1篇论文）
免疫荧光分析（1篇论文）
外觀
1 mg/mL 溶液（溶于 0.1 M 磷酸钠、0.1 MNaCl，pH 7.5，含 5 mM 叠氮化钠作为防腐剂）中提供了 1 mL 单位规格的 Atto 647 羊抗兔 IgG（全分子）。
分析報告
非结合染料≤总荧光的 5%
法律資訊
本产品仅供研究使用。如果打算商业化，请联系知识产权持有者（德国ATTO-TEC GmbH公司）申请许可。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Till F M Andlauer et al.
eLife, 3 (2014-11-14)
CIDE-N domains mediate interactions between the DNase Dff40/CAD and its inhibitor Dff45/ICAD. In this study, we report that the CIDE-N protein Drep-2 is a novel synaptic protein important for learning and behavioral adaptation. Drep-2 was found at synapses throughout the
Giuliana Fusco et al.
Nature communications, 7, 12563-12563 (2016-09-20)
α-synuclein (αS) is an intrinsically disordered protein whose fibrillar aggregates are the major constituents of Lewy bodies in Parkinson's disease. Although the specific function of αS is still unclear, a general consensus is forming that it has a key role
Anna Albisetti et al.
PLoS pathogens, 13(11), e1006710-e1006710 (2017-11-02)
Trypanosoma brucei belongs to a group of unicellular, flagellated parasites that are responsible for human African trypanosomiasis. An essential aspect of parasite pathogenicity is cytoskeleton remodelling, which occurs during the life cycle of the parasite and is accompanied by major
Masfique Mehedi et al.
Bio-protocol, 7(17) (2017-10-24)
Human respiratory syncytial virus (RSV) infection in human lung epithelial A549 cells induces filopodia, cellular protrusions consisting of F-actin, that extend to neighboring uninfected cells (Mehedi et al., 2016). High-resolution imaging via stimulated emission depletion (STED) microscopy revealed filamentous RSV
Intracellular repair of oxidation-damaged α-synuclein fails to target C-terminal modification sites.
Andres Binolfi et al.
Nature communications, 7, 10251-10251 (2016-01-26)
Cellular oxidative stress serves as a common denominator in many neurodegenerative disorders, including Parkinson's disease. Here we use in-cell NMR spectroscopy to study the fate of the oxidation-damaged Parkinson's disease protein alpha-synuclein (α-Syn) in non-neuronal and neuronal mammalian cells. Specifically
文章
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
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