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48275

Sigma-Aldrich

β 半乳糖苷酶 来源于大肠杆菌

suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg

同義詞:

β-D-半乳糖苷半乳糖水解酶, 乳糖酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

powder

品質

lyophilized

比活性

~140 U/mg

分子量

Mr ~135000

包含

phosphate buffer as stabilizer
sucrose as stabilizer

技術

enzyme immunoassay: suitable

運輸包裝

wet ice

儲存溫度

−20°C

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相關類別

應用

水溶液可以直接用于衍生化,而无需事先进行透析或凝胶渗透色谱法。

生化/生理作用

β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。

包裝

无底玻璃瓶。内含物装在插入的融合锥内。

物理性質

四聚体的分子量为465 kDa(每个亚基116.3 kDa)

單位定義

1U对应于在pH 7.8和37℃下每分钟水解1 μmol 2-硝基苯基-β-D-吡喃半乳糖苷(目录号73660)所需的酶量。

其他說明

用于具有4-甲基伞形酮基-β-D-半乳糖苷(目录号69590)的人IgG的高灵敏度酶联免疫测定;夹心酶联免疫分析技术评价β-D-半乳糖苷酶(大肠埃希菌)和辣根过氧化物酶标记物;促甲状腺素释放激素(TRH)的酶联免疫测定;酶-抗体偶联物的制备

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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M Takeyama et al.
Chemical & pharmaceutical bulletin, 40(8), 2199-2201 (1992-08-01)
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled beta-D-galactosidase (beta-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH2)6-NH2 (TRH-Hex), was employed. TRH-Hex was linked to beta-gal
K Kato et al.
European journal of biochemistry, 62(2), 285-292 (1976-02-16)
Rabbit immunoglobulin G (IgG) was reduced by incubating with 10 mM 2-mercaptoethylamine and then treated with excess amounts of N,N'-o-phenylenedimaleimide. As a result, maleimide residues were introduced into rabbit IgG molecules. Rabbit IgG containing maleimide residues could be coupled to
M Imagawa et al.
Annals of clinical biochemistry, 21 ( Pt 4), 310-317 (1984-07-01)
beta-D-galactosidase from Escherichia coli and horseradish peroxidase were evaluated as labels of Fab' in dose-response curves for human alpha-fetoprotein and human chorionic gonadotropin by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The non-specific binding of Fab'-peroxidase conjugates
Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay.
M J O'Sullivan et al.
Analytical biochemistry, 100(1), 100-108 (1979-11-15)
Christophe Laumonnerie et al.
Development (Cambridge, England), 141(3), 594-603 (2014-01-23)
The establishment of anatomically stereotyped axonal projections is fundamental to neuronal function. While most neurons project their axons within the central nervous system (CNS), only axons of centrally born motoneurons and peripherally born sensory neurons link the CNS and peripheral

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