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Key Documents

43817

Sigma-Aldrich

DL-二硫苏糖醇

BioUltra, ≥99.0% (RT)

同義詞:

1,4-二巯基苏糖醇, DTT, 克莱兰氏试剂

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About This Item

線性公式:
HSCH2CH(OH)CH(OH)CH2SH
CAS號碼:
分子量::
154.25
Beilstein:
1719757
EC號碼:
MDL號碼:
分類程式碼代碼:
12352200
PubChem物質ID:

產品線

BioUltra

化驗

≥99.0% (RT)

形狀

solid

雜質

insoluble matter, passes filter test
≤0.3% 4,5-dihydroxy-1,2-dithiane

pH值

4.0-6.5 (25 °C, 0.1 M in H2O)

mp

41-44 °C (lit.)

溶解度

H2O: 0.1 M at 20 °C, clear, colorless

負離子痕跡

sulfate (SO42-): ≤50 mg/kg

正離子痕跡

Al: ≤5 mg/kg
As: ≤0.5 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤100 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

&lambda ;

0.1 M in H2O

紫外吸收

λ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100

儲存溫度

2-8°C

SMILES 字串

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI 密鑰

VHJLVAABSRFDPM-QWWZWVQMSA-N

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應用

用于将 −SH 基保持在还原态的绝佳试剂;定量还原二硫化物。在 SDS-PAGE 前,DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常,使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。

其他說明

Cleavage of disulfide bonds in proteins; In the investigation of incremental and kinetic reduction in proteins; Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues; In protein sequence analysis.

象形圖

CorrosionExclamation mark

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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A.Bacarese-Hamilton et al.
Annals of the New York Academy of Sciences, 448, 571-571 (1985)
D W Sears et al.
Biochemistry, 16(9), 2031-2035 (1977-05-03)
The reduction by dithiothreitol (DTT) of the four interchain disulfides of a human IgGlkappa immunoglobulin has been studied by two methods: variation of the concentration of DTT relative to the protein concentration (incremental reduction); and variation of the time of
J R Jabusch et al.
Analytical biochemistry, 91(2), 532-542 (1978-12-01)
The thiazolinone and phenylthiohydantoin derivatives of most amino acids can be hydrolyzed with alkaline dithionite to generate the free amino acid. The acidification of this hydrolysate with 3 N HCl containing thiodiglycol leads in the case of glutamic acid, glutamine
Reductive cleavage of cystine disulfides with tributylphosphine.
U T Rüegg et al.
Methods in enzymology, 47, 111-116 (1977-01-01)
Marija Smokvarska et al.
Current biology : CB, 30(23), 4654-4664 (2020-10-10)
In the course of their growth and development, plants have to constantly perceive and react to their environment. This is achieved in cells by the coordination of complex combinatorial signaling networks. However, how signal integration and specificity are achieved in

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