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01673

Sigma-Aldrich

吖啶黄

suitable for fluorescence, BioReagent, ≥90% (AT)

同義詞:

中性吖啶黄素, 优黄素, 氯代3,6-二氨基-10-甲基吖啶和3,6-二氨基吖啶（原黄素）混合物

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About This Item

經驗公式（希爾表示法）:
C14H14ClN3
CAS號碼:
8048-52-0
分子量：:
259.73
MDL號碼:
MFCD00064307
分類程式碼代碼:
12171500
PubChem物質ID:
329747342
NACRES:
NA.32

產品線

BioReagent

化驗

≥90% (AT)

形狀

powder

成份

Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC

溶解度

H2O: 0.33 g/mL (lit.)(lit.)

螢光

λex 463 nm; λem 490 nm in ethanol

適合性

suitable for fluorescence

SMILES 字串

[Cl-].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]4c5cc(N)ccc5cc6ccc(N)cc46

InChI

1S/C14H13N3.C13H11N3.ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;1H

InChI 密鑰

PEJLNXHANOHNSU-UHFFFAOYSA-N

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應用

吖啶黄用于荧光标记高分子量 RNA 分子 。
各种溶剂中的激发和发射波长 ：
  • 甲醇：λex = 424 nm；λem = 518 nm
  • 乙醇：λex = 426 nm；λem = 524 nm
  • 丙醇：λex = 430 nm；λem = 512 nm
  • 丁醇：λex = 430 nm；λem = 526 nm
  • 甲酰胺：λex = 434 nm；λem = 524 nm
  • 甘油：λex = 432 nm；λem = 540 nm
  • 水：λex = 416 nm；λem = 514 nm

不溶于乙醚、氯仿和固定油。在荧光稳态测量中用作供体分子（当与罗丹明 6G 作为受体配对时），起到 pH 传感器的作用 。

其他說明

DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously

象形圖

Exclamation markEnvironment
GHS07,GHS09

訊號詞

Warning

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

分析證明 (COA)

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M Stöhr et al.
Stain technology, 53(4), 205-215 (1978-07-01)
Eight fluorescent dye combinations for simultaneous DNA-protein staining have been evaluated spectroscopically and flow microfluorometrically: propidium iodide (PI) with fluoresceinisothiocyanate (FITC), fluorescamine (FC), and dansylchloride (DANS); diamidinophenylindole (DAPII) with sulphorhodamin (SR101), tetramethylrhodamin isothiocyanate (TRITC), and nitrobenzodiazole (NBD); acriflavine (AF) with
Zheqing Cai et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(43), 17462-17467 (2013-10-09)
Both preclinical and clinical studies suggest that brief cycles of ischemia and reperfusion in the arm or leg may protect the heart against injury following prolonged coronary artery occlusion and reperfusion, a phenomenon known as remote ischemic preconditioning. Recent studies
Ivan Gusarov et al.
Science (New York, N.Y.), 325(5946), 1380-1384 (2009-09-12)
Bacterial nitric oxide synthases (bNOS) are present in many Gram-positive species and have been demonstrated to synthesize NO from arginine in vitro and in vivo. However, the physiological role of bNOS remains largely unknown. We show that NO generated by
Nadine Fischer et al.
Biochimica et biophysica acta, 1828(2), 632-641 (2012-10-24)
Acriflavine resistance protein B acts as the active transporter in the multi-drug efflux pump Acriflavine resistance proteins A / B - Tolerance to colicins protein in Escherichia coli. Within the same reaction cycle intermediate all Acriflavine resistance protein B X-ray
Jessica E S Shay et al.
Carcinogenesis, 35(5), 1067-1077 (2014-01-11)
Hypoxia-inducible factors (HIFs) accumulate in both neoplastic and inflammatory cells within the tumor microenvironment and impact the progression of a variety of diseases, including colorectal cancer. Pharmacological HIF inhibition represents a novel therapeutic strategy for cancer treatment. We show here
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