PPB002

Tris Buffered Saline

powder, pH 8.0, pHast Pack^™, with 0.05% Tween^® 20

• 同義詞: Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST
• 分類程式碼代碼: 41105323
• NACRES: NA.21

屬性

• 產品名稱: Tris 缓冲盐水，含Tween ™ 20，pH 8.0, powder, pHast Pack^™
• 產品線: pHast Pack^™
• 形狀: powder
• 儲存條件: (Tightly closed. Dry.)
• 雜質: ≤5 ppm Heavy Metals (lead)
• 顏色: white
• pH值: 7.8—8.2
• 溶解度: soluble, clear to hazy, colorless
• 正離子痕跡: Fe: ≤10 ppm
• 異物活動: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• 儲存溫度: room temp

描述

一般說明

Tris Buffered Saline with 0.05% Tween^® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.

應用

Suitable for use as a wash buffer in:

• Western blot
• ELISA
• Immunohistochemistry

特點和優勢

• Ready-to-use buffer requires no measurments or pH adjustments
• Saves time, money and effort to get your assays started fast
• Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
• Qualified for use in gel electrophoreis and other molecular biology assays

包裝

Foil pouches

重構

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

儲存和穩定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

其他說明

For additional information on our range of Biochemicals, please complete this form.

法律資訊

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable