推薦產品
產品名稱
磷酸盐缓冲剂，含0.05% TWEEN® 20，pH 7.4,
形狀
powder
溶解度
water: soluble, clear, colorless (1 pouch dissolved in 1L)
應用
cell analysis
diagnostic assay manufacturing
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一般說明
磷酸盐缓冲液-Tween®20(PBST)主要用作免疫分析中的洗涤和封闭缓冲液。磷酸缓冲液用于维持稳定的pH 7.4，适合许多生物反应和试验。PBS-T中的盐或生理盐溶液用于提供维持最佳蛋白溶解度和稳定性需要的离子强度。0.05% TWEEN®-20是一种非离子去污剂，用于通过封闭蛋白的非特异性结合位点降低背景信号。PBST缓冲液通过清除不必要物质提高洗涤效率，并在保持靶蛋白完整性的前提下控制非特异性抗体结合位点。
應用
磷酸盐缓冲液用于：
- 蛋白质免疫印迹分析
- 酶联免疫吸附测定(ELISA)
- 端粒FISH (Tel-FISH)和端粒/免疫染色-FISH (Teli-Fish)
- 细胞分离
包裝
锡箔袋包装
重構
一个袋中的内容物溶解于1L蒸馏水或超纯水水中，将得到0.01 M磷酸盐缓冲液、0.138M NaCl、0.0027M KCl、0.05% Tween 20，pH7.4.
法律資訊
TWEEN is a registered trademark of Croda International PLC
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.
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