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Merck
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重要文件

69966

Millipore

MRS肉汤

suitable for microbiology, NutriSelect® Basic

同義詞:

乳酸杆菌肉汤(根据De Man,Rogosa和Sharpe的报道)

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About This Item

分類程式碼代碼:
41171607
NACRES:
NA.74

無菌

non-sterile

品質等級

形狀

powder

儲存期限

limited shelf life, expiry date on the label

成份

dipotassium hydrogen phosphate, 2 g/L
glucose, 20 g/L
magnesium sulfate monohydrate, 0.2 g/L
manganous sulfate tetrahydrate, 0.05 g/L
meat extract, 8 g/L
peptone, 10 g/L
sodium acetate, 5 g/L
triammonium citrate, 2 g/L
yeast extract, 4 g/L

包裝

pkg of 500 g

製造商/商標名

NutriSelect® Basic

技術

microbiological culture: suitable

pH值

6.2±0.2

應用

agriculture
bioburden testing
environmental
food and beverages

microbiology

適合性

selective by low pH for Bifidobacterium spp.
selective by low pH for Lactobacillus spp.

一般說明

MRS 肉汤用于从所有类型的材料中富集、培养和分离所有种类的乳酸杆菌(根据 DeMan、Rogosa 和 Sharpe 的报道)。该培养基可用于从 MRS 琼脂中分离出的菌落的确认测试。MRS 培养基含有聚山梨醇酯(Tween 80)、醋酸盐、镁和锰,这些都是乳酸杆菌的已知的特殊生长因子,并且是丰富的营养基础。由于这些培养基显示出非常低的选择性,片球菌明串珠菌以及其他继发细菌可能会在其中生长。大多数伴随的微生物群落可以被醋酸铊、山梨酸、醋酸、亚硝酸钠、放线菌素和多粘菌素抑制。这些物质可以以不同的浓度和组合使用,但不可避免地必须在有机体的选择性和回收率之间达成妥协。

應用

MRS 肉汤用于从所有类型的材料中富集、培养、分离所有种类的乳酸杆菌

準備報告

将 51.25 g 溶解在 1 升蒸馏水中并加入 1 ml Tween® 80(货号 P8074)。加热煮沸至完全溶解培养基。装入容器并通过121°C高压灭菌15 分钟进行灭菌。在 35°C 下孵育培养物最多3天,或在30°C下最多孵育 5 天。如果可能,在富含二氧化碳的厌氧罐中孵育培养物。

法律資訊

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

免責聲明

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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L.C. Laleye et al.
Journal of Food Protection, 50, 1009-1009 (1987)
P Laloi et al.
Applied microbiology and biotechnology, 36(2), 196-204 (1991-11-01)
Whole cells of Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 were able to hydrolyse alpha- and beta-caseins. Irrespective of the growth medium used, milk or De Man-Rogosa-Sharpe (MRS) broth, identical patterns of alpha- and beta-casein hydrolytic products, respectively, were visualized by
J.D. DeMan et al.
The Journal of Applied Bacteriology, 23, 130-130 (1960)
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes

文章

Molecular biology-based methods, like PCR, can be used for lactobacilli detection.

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

probiotics-and-human

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