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39465

Millipore

明胶 来源于猪皮肤

suitable for microbiology, ultrahigh gel strength

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
41106212
NACRES:
NA.85

生物源

Porcine

品質等級

形狀

powder

品質

ultrahigh gel strength

儲存期限

limited shelf life, expiry date on the label

損耗

9.5-12.5% loss on drying

透射率

450 nm, ≥85%
620 nm, ≥95%

pH值

5.20-5.60

傅導性

120-190 μS/cm at 30 °C (1%)

黏度

5.10-5.80 mPa.s

凝膠強度

280-302 g BloomAOAC

溶解度

H2O: 67 mg/mL at 60 °C

正離子痕跡

Ca: ≤100 mg/kg

應用

microbiology

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應用

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

成分

明胶存在于胶原蛋白中,是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

注意

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

準備報告

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentrat ion. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. Manufactured by Gelita AG

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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M V Nasekin et al.
Anesteziologiia i reanimatologiia, (1)(1), 59-60 (2013-07-03)
The article deals with skills improving problem of epidural anaesthesia with ultrasound control. Methods of gelatin spinal column model making, use and its economical side are discussed in the article.
Johno Breeze et al.
Journal of the Royal Army Medical Corps, 159(2), 84-89 (2013-05-31)
Ballistic gelatin is the most common tissue simulant used to reproduce the penetration of projectiles into muscle but published data to support its use are primarily based on bullets, despite explosive fragments being the most common cause of injury to
Takeo Furuya et al.
The journal of spinal cord medicine, 36(2), 134-139 (2013-07-03)
Besides stimulating angiogenesis or cell survival, basic fibroblast growth factor (bFGF) has the potential for protecting neurons in the injured spinal cord. To investigate the effects of a sustained-release system of bFGF from gelatin hydrogel (GH) in a rat spinal
Monica A Serban et al.
Methods in molecular biology (Clifton, N.J.), 1001, 133-143 (2013-03-16)
Tissue engineering involves the concerted action of biomaterials, cells, and growth factors. Kidney -regeneration relies on the same combination of ingredients. Here, we describe an example of gelatin-based biomaterial preparation and its evaluation in the context of kidney biocompatibility and
Nina E Lamash et al.
PloS one, 8(3), e58433-e58433 (2013-03-19)
Four proteases with molecular masses of 132, 58, 53, and 47 kDa were detected in the digestive system of the holothurian Eupentacta fraudatrix. These proteases displayed the gelatinase activity and characteristics of zinc metalloproteinases. The 58 kDa protease had similar

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