PFLRKB

KAPA PROBE FAST

suitable for qPCR, 2 ×, ROX^™ Low

• 同義詞: Probe qPCR, qPCR
• 分類程式碼代碼: 41106300
• NACRES: NA.55

屬性

• 品質等級: 100
• 用途: sufficient for 100 reactions; sufficient for 1000 reactions; sufficient for 500 reactions
• 儲存期限: ≤12 mo.
• 特點: dNTPs included; hotstart
• 包裝: kit of 1 mL (100 x 20 μL rxn; KK4716); kit of 10 mL (1000 x 20 μL rxn; KK4718); kit of 5 mL (500 x 20 μL rxn; KK4717); pkg of
• 製造商／商標名: Roche
• 濃度: 2 ×
• 技術: qPCR: suitable
• 輸入: purified DNA
• 相容性: for use with Agilent Mx3000P; for use with Agilent Mx3005P; for use with Agilent Mx4000; for use with Applied Biosystems^® 7500; for use with Applied Biosystems^® ViiA 7; for use with QuantStudio^™
• 檢測方法: probe-based
• 儲存溫度: −20°C

描述

一般說明

KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan^®), FRET probes, and displacement probes (e.g. molecular beacons).

應用

KAPA PROBE FAST qPCR Master Mix (2X) with ROX^™ Low is suitable for use in:

• Genotyping
• Gene expression analysis
• Multiplex qPCR
• SNP genotyping/allelic discrimination
• Microarray validation
• Real time(RT)-quantitative polymerase chain reaction (qPCR)/qPCR

特點和優勢

Accurately discriminate between alleles:

• Exceptional differentiation of heterozygous and homozygous alleles
• Discrete clusters in SNP genotyping assays

Achieve excellent reproducibility and efficiency:

• Fast, high performance five-colored multiplex PCR
• Similar abundance levels achieved with low copy number
• Broad dynamic range of up to 10 orders of magnitude

Increase flexibility:

• Compatible with both standard and fast cycling protocolsRetain accurate quantification

Quick Notes:

• These kits are compatible with all fluorogenic probebased technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan^®) and displacement probes (e.g., molecular beacons).
• Initial denaturation for 20 sec at 95ºC is sufficient for enzyme reactivation; however, optimal denaturation of complex targets may require up to 3 min denaturation.
• For 2-step cycling, use 30 sec combined annealing/ extension/data acquisition.
• For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition.
• Do not exceed 25 μL volumes.

品質

KAPA PROBE FAST qPCR Master Mix (2X) is subjected to stringent quality control tests, is free of contaminating exoand endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

準備報告

ROX/fluorescein reference dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. Avoid repeated freezing and thawing. Always ensure that the product has been fully thawed and mixed before use.

KAPA PROBE FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

其他說明

For Research Use Only. Not for use in diagnostic procedures.

法律資訊

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

QuantStudio is a trademark of IROA Technologies LLC

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

僅套裝組件

• KAPA PROBE FAST qPCR Master Mix (2X) with ROX^™ Low
• KAPA Taq HotStart^® DNA Polymerase
• reaction buffer
• dNTPs
• MgCl₂ 5 nM

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: does not flash
• 閃點（°C）: does not flash