11759779001

BM Chemiluminescence ELISA Substrate (AP)

• 分類程式碼代碼: 12352204

屬性

• 用途: sufficient for 1,000 tests (ELISA); sufficient for 600 tests (SEAP)
• 包裝: pkg of 150 mL
• 製造商／商標名: Roche
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C

描述

特異性

The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).

特點和優勢

• Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
• High dynamic measuring range: Linear range of more than five orders of magnitude
• Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics

成分

1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer

原則

CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.

準備報告

Working solution: Preparation of working solution

The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.

其他說明

仅用于生命科学研究。不可用于诊断。