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03353575910

Roche

DIG寡核苷酸3′-末端标记试剂盒,第2代

greener alternative

sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide), storage condition avoid repeated freeze/thaw cycles

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About This Item

分類程式碼代碼:
41105500
NACRES:
NA.55

用途

sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide)

品質等級

製造商/商標名

Roche

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

環保替代類別

運輸包裝

dry ice

一般說明

我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。  DIG系统是灵敏且具有成本效益的方案,可替代放射性标记和放射法检测核酸。有许多已发表论文证明了DIG系统的通用性,因此,使用放射性标记不再是标记用于杂交的DNA的唯一选择。
第二代寡核苷酸3′末端地高辛标记试剂盒使用了末端转移酶,可将单个地高辛标记双脱氧尿苷三磷酸(ddUTP)催化添加到寡核苷酸的3′-OH端上,从而标记寡核苷酸。

應用

第二代寡核苷酸3′末端地高辛标记试剂盒采用DIG-11-ddUTP和重组末端转移酶对14-100bp的寡核苷酸进行3′-末端标记。
DIG标记寡核苷酸已用于多种杂交技术:
  • 斑点/狭缝印迹
  • 菌落/斑块杂交
  • Southern印迹/Northern印迹
  • 原位杂交

特點和優勢

  • 由于寡核苷酸具有小分子量,可用于快速杂交动力学
  • 单链探针,在杂交过程中不会复性
  • 可以根据实验设计序列
  • 特别适用于原位杂交;由于寡核苷酸具有小分子量,因此易于扩散到固定的组织和细胞中

包裝

1个试剂盒包含9个组分。

品質

已通过斑点杂交试验测试功能。

原則

利用重组末端转移酶将一个DIG-ddUTP分子添加到寡核苷酸的3′-末端。这能够确保获得具有高特异性和独特性的杂交信号,该信号可利用酶联免疫测定结合抗DIG-AP抗体偶联物以及一种有色或化学发光反应进行检测。

準備報告

活化剂:硫酸钠,Tris
工作浓度:寡核苷酸:100 pmol
在一次标准标记反应中,最多能够标记100pmol(一个30聚体为1 μg)寡核苷酸。
测定时间:从标记寡核苷酸到杂交和检测到第一个可见信号的完整过程,可在24小时内完成。

样品材料
长度为14至100个核苷酸的寡核苷酸,经过HPLC或凝胶电泳纯化

儲存和穩定性

-15–-25 °C储存。(未开封试剂盒)

其他說明

仅用于生命科学研究。不可用于诊断。

僅套裝組件

產品號碼
描述

  • Reaction Buffer 5x concentrated

  • CoCl<sub>2</sub> Solution 25 mM

  • DIG-ddUTP Solution 1 mM

  • Recombinant Terminal Transferase 400 U/μl

  • Control Oligonucleotide, unlabeled 20 pmol/μl

  • Oligonucleotide, DIG-ddUTP labeled 2.5 pmol/μl

  • Control DNA, 2.5 pmol/μl pUC 18 DNA, supercoiled

  • Glycogen Solution 20 mg/ml

  • DNA Dilution Buffer, 50 μg/ml fish sperm DNA

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訊號詞

Danger

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B Inhalation - Repr. 1B

儲存類別代碼

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

does not flash

閃點(°C)

does not flash


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Ming-Xiang Zhang et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(47), 16967-16972 (2005-11-15)
Repeats (27-nt) in intron 4 have been shown to play a cis-acting role in endothelial nitric oxide synthase (eNOS) promoter activity. We hypothesize that the 27-nt repeats could be the source of small nuclear RNA specifically regulating eNOS expression. In
Pieter J Oort et al.
Molecular and cellular endocrinology, 276(1-2), 24-35 (2007-08-11)
Tumor suppressor candidate 5 (Tusc5, also termed brain endothelial cell derived gene-1 or BEC-1), a CD225 domain-containing, cold-repressed gene identified during brown adipose tissue (BAT) transcriptome analyses was found to be robustly-expressed in mouse white adipose tissue (WAT) and BAT
Seong Su Kang et al.
Translational stroke research, 4(5), 533-545 (2013-12-07)
Increasing endogenous ciliary neurotrophic factor (CNTF) expression with a pharmacological agent might be beneficial after stroke as CNTF both promotes neurogenesis and, separately, is neuroprotective. P2X7 purinergic receptor inhibition is neuroprotective in rats and increases CNTF release in rat CMT1A
Richard Park et al.
Journal of virology, 92(20) (2018-08-03)
Profound alterations in host cell nuclear architecture accompany the lytic phase of Epstein-Barr virus (EBV) infection. Viral replication compartments assemble, host chromatin marginalizes to the nuclear periphery, cytoplasmic poly(A)-binding protein translocates to the nucleus, and polyadenylated mRNAs are sequestered within
Omer Choresh et al.
Biomacromolecules, 10(10), 2852-2856 (2009-09-08)
The various silks that make up the web of the orb web spiders have been studied extensively. However, success in prey capture depends as much on the web glue as on the fibers. Spider silk glue, which is considered one

文章

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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