推薦產品
運輸包裝
dry ice
品質等級
一般說明
The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.
CET 1019 HS-hygro-SceI is a mammalian expression plasmid vector containing the mouse Rps3 UCOE upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.
CET 1019 AS-hygro-SceI is a mammalian expression plasmid vector containing the murine Rps3 UCOE upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.
The CET 1019 HS-hygro-SceI and CET 1019 AS-hygro-SceI vectors possess the following features:
THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.
CET 1019 HS-hygro-SceI is a mammalian expression plasmid vector containing the mouse Rps3 UCOE upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.
CET 1019 AS-hygro-SceI is a mammalian expression plasmid vector containing the murine Rps3 UCOE upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.
The CET 1019 HS-hygro-SceI and CET 1019 AS-hygro-SceI vectors possess the following features:
- A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
- A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
- A hygromycin antibiotic resistance gene for selection of stably transfected mammalian cells
- A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
- An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes
THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.
應用
Research Category
General Cell Biology
All
General Cell Biology
All
Research Sub Category
All
All
Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression by removing epigenetic related gene silencing.
成分
1) 10ug CET 1019 HS-hygro-SceI (+) Vector (CS221288)
2) 10ug CET 1019 AS-hygro-SceI (+) Vector (CS221289)
3) 10ug SC HS-hygro (-) Vector (CS221290)
4) 10ug SC AS-hygro (-) Vector (CS221291)
2) 10ug CET 1019 AS-hygro-SceI (+) Vector (CS221289)
3) 10ug SC HS-hygro (-) Vector (CS221290)
4) 10ug SC AS-hygro (-) Vector (CS221291)
儲存和穩定性
Plasmids should be stored at -20C. Avoid repeated freeze-thaw events.
其他說明
Concentration: Please refer to lot specific datasheet.
法律資訊
UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
Genomics, 82(3), 269-279 (2003-08-09)
The genetic elements that are responsible for establishing a transcriptionally competent, open chromatin structure at a region of the genome that consists only of ubiquitously expressed, housekeeping genes are currently unknown. We demonstrate for the first time through functional analysis
我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務