SCT064
BioTracker NTP-Transporter Molecule
Quick and efficient transport of labeled NTPs into cells for live cell imaging of DNA replication, synthesis, etc. Labeling of DNA with fluorescent NTPs is one of the essential methods in cell cycle analysis.
同義詞:
Cell cycle detection, Live cell imaging probe, Live cell imaging probe, Cell cylce probe
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About This Item
分類程式碼代碼:
12352207
NACRES:
NA.47
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化驗
≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)
技術
cell based assay: suitable
檢測方法
fluorometric
一般說明
Nucleoside triphosphates (NTPs) are important metabolites that are involved in numerous cellular processes. However, due to their anionic nature, NTPs do not readily permeate cell membranes. To overcome this barrier, synthetic analogues that are neutral and more readily transportable are commonly used in cell-based assays. However, these synthetic analogues have a very low efficiency of conversion to active metabolites. Hence there is a need to optimize transport of NTPs into the cells.
BioTracker<TMSYMBOL></TMSYMBOL> NTP-Transporter Molecule has a receptor site, for selectively binding to the NTPs, and a cell penetrating agent. The transporter can bind to NTPs and quickly move them into cells without any apparent damage to the cell membrane or decrease in cell viability. The transported NTP is rapidly liberated from the complex and available in the nuclei for incorporation into DNA. Labeled NTPs can be used for live cell imaging, cell proliferation assays, synthetic biology studies, synthesizing artificial DNA, tracking cell division, research on active antiviral and anticancer nucleotides, among other things.
BioTracker<TMSYMBOL></TMSYMBOL> NTP-Transporter Molecule has a receptor site, for selectively binding to the NTPs, and a cell penetrating agent. The transporter can bind to NTPs and quickly move them into cells without any apparent damage to the cell membrane or decrease in cell viability. The transported NTP is rapidly liberated from the complex and available in the nuclei for incorporation into DNA. Labeled NTPs can be used for live cell imaging, cell proliferation assays, synthetic biology studies, synthesizing artificial DNA, tracking cell division, research on active antiviral and anticancer nucleotides, among other things.
應用
Live cell fluorescent imaging
Quick and efficient transport of labeled NTPs into cells for live cell imaging of DNA replication, synthesis, etc. Labeling of DNA with fluorescent NTPs is one of the essential methods in cell cycle analysis.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Cell Cycle, DNA Replication & Repair
DNA Damage & Repair
Cell Cycle, DNA Replication & Repair
DNA Damage & Repair
品質
Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 3470.14 g/mol
Molar Mass: 3470.14 g/mol
外觀
Lyophilized
儲存和穩定性
Store BioTracker NTP-Transporter Molecule at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Zbigniew Zawada et al.
Angewandte Chemie (International ed. in English), 57(31), 9891-9895 (2018-03-27)
Chemically modified nucleoside triphosphates (NTPs) are widely exploited as unnatural metabolites in chemical biology and medicinal chemistry. Because anionic NTPs do not permeate cell membranes, their corresponding neutral precursors are employed in cell-based assays. These precursors become active metabolites after
文章
The NTP transporter allows dUTPs to enter live cells and is an alternative to BrdU/EdU cell proliferation assays used to measure cell cycle dynamics in real-time.
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