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SCM103

Sigma-Aldrich

EmbryoMax® MEF Conditioned Media

EmbryoMax MEF Conditioned Media (MEF-CM) can be used for the feeder-free culture of human ES/iPS cells.

同義詞:

MEFCM, MEF-CM, Mouse Embryonic Fibroblast Conditioned Media

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About This Item

分類程式碼代碼:
41106514
eCl@ss:
32011203
NACRES:
NA.71

品質等級

製造商/商標名

Specialty Media
EmbryoMax®

技術

cell culture | stem cell: suitable

運輸包裝

ambient

一般說明

Traditionally, human embryonic stem cell (hESC) or induced pluripotent stem cell (iPSC) culture require mouse embryonic fibroblasts (MEF) support. This requirement makes the protocol complicated and variable. MEF conditioned medium (MEFCM) contains all the growth factors needed in hESC/iPSC culture, eliminating the routine preparation of feeder cells. It is a ready-to-use product for the feeder-free culture of human ES/IPS cells.

Each batch of EmbryoMax MEF Conditioned Media has been tested for hES/iPS cells pluripotency. The media requires an additional 10 ng/ml of bFGF to be added once thawed before use.

細胞系描述

Primary Mouse Embryo Fibroblasts

品質

Mycoplasma Testing: PASSED
Bacterial Testing: PASSED
Fungi Testing: PASSED

儲存和穩定性

Store at -80C until expiration date on label.

法律資訊

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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條款

产生人诱导多能干细胞(iPSC)的干细胞重编程实验方案,包括基于病毒和非病毒RNA的方法。

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