SCC291

SHSY-5Y Tet-On Inducible Alpha-Synuclein Neuroblastoma Cell Line

Human

• 同義詞: SHSY-5Y Tet-On Inducible Alpha-Synuclein Neuroblastoma Cell Line
• 分類程式碼代碼: 41106514
• NACRES: NA.81

屬性

• 產品名稱: SHSY-5Y Tet-On Inducible Alpha-Synuclein Neuroblastoma Cell Line,
• 生物源: human
• 包裝: pkg of >1X10⁶ vials
• 製造商／商標名: Sigma-Aldrich
• 增長模式: N/A
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: ≤ − 140°C

描述

一般說明

The SHSY-5Y Tet-ON inducible α-synuclein neuroblastoma cell line serves as a model for both dopaminergic neuronal biology and α-synuclein pathology. SHSY-5Y cells may be induced upon treatment with retinoic acid to differentiate into neuronal-type cells, enabling studies of neuronal function and differentiation.4 SHSY-5Y cells secrete catecholamines and thus serve as a model for dopaminergic neurons. The SHSY-5Y TetON α-synuclein inducible cell line harbors an α-synuclein construct inducible by treatment with doxycycline, thus allowing controlled overexpression of α-synuclein before or after neuronal differentiation.5 The construct also includes a tet repressor, which prevents leaky expression in absence of inducer. This unique cell line is an excellent model for both the basic cellular physiology of Parkinson′s disease and therapeutic development.

應用

Parkinson′s disease, the most common neurodegenerative movement disorder1, is caused by the progressive degeneration of dopaminergic neurons. A signature pathology of the brains of Parkinson′s patients is the buildup of misfolded aggregates of the protein α-synuclein.2 Aggregates of α-synuclein are also implicated in Lewy body dementia and Alzheimer′s disease.3 Although study of the complete pathogenesis of neurodegenerative diseases such as Parkinson′s requires animal models, cellular models that reflect distinct aspects of Parkinson′s pathology are of great value for understanding the multifaceted character of neurodegenerative diseases and useful for developing targeted treatments.The SHSY-5Y Tet-ON inducible α-synuclein neuroblastoma cell line serves as a model for both dopaminergic neuronal biology and α-synuclein pathology. SHSY-5Y cells may be induced upon treatment with retinoic acid to differentiate into neuronal-type cells, enabling studies of neuronal function and differentiation.4 SHSY-5Y cells secrete catecholamines and thus serve as a model for dopaminergic neurons. The SHSY-5Y TetON α-synuclein inducible cell line harbors an α-synuclein construct inducible by treatment with doxycycline, thus allowing controlled overexpression of α-synuclein before or after neuronal differentiation.5 The construct also includes a tet repressor, which prevents leaky expression in absence of inducer. This unique cell line is an excellent model for both the basic cellular physiology of Parkinson′s disease and therapeutic development.Source The SHSY-5Y Tet-ON Inducible -Synuclein cell line is stably transfected with a construct containing an alpha-synuclein cDNA cassette and CMV promoter under control of Tet-ON response element.5 The parental SHSY-5Y cell line was derived from a metastatic neuroblastoma of a 4-year-old female.6References1. Falkenburger BH, Saridaki T, Dinter E. J Neurochem 2016;139 (Suppl 1):121-130.2. Balestrino R, Schapira AHV. Eur J Neurol 2020; 27(1):27-42.3. Twohig D, Nielsen HM. Mol Neurodegener 2019; 14(1):23. 4. Agholme L et al. J Alzheimer′s Dis 2010; 20(4):1069-1082.5. Vasquez V, Mitra J, Perry G, Rao KS, Hegde ML. J Alzheimer′s Dis 2018; 66(2):453-460.6. Biedler JL, Helson L, Spengler BA. Cancer Res 1973; 33(11):2643-2652.

• Each vial contains ≥ 1X10⁶ viable cells.• SHSY-5Y Tet-ON Inducible -Synuclein Neuroblastoma cells are verified to be of human origin and negative for mouse, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services • Cells tested negative for infectious diseases against a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.• Cells tested negative for mycoplasma.

特點和優勢

SHSY-5Y Tet-ON inducible α-synuclein neuroblastoma cell line serves as a model for both dopaminergic neuronal biology and α-synuclein pathology.

儲存和穩定性

The cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

其他說明

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable