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重要文件

S7806

Sigma-Aldrich

CpG WIZ Prader-Willi/Angelman -Methylation specific PCR assay

The CpG WIZ Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation-specific PCR (MSP).

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.31

品質等級

物種活性

human

製造商/商標名

CPGWIZ
Chemicon®

技術

PCR: suitable

NCBI登錄號

UniProt登錄號

應用

genomic analysis

運輸包裝

dry ice

基因資訊

human ... NIPA1(123606)

相關類別

一般說明

A differentially methylated site exists in the CpG islands of SNRPN (small nuclear ribonucleoprotein-associated polypeptide N), a candidate gene for Prader-Willi/Angelman Syndrome. Normally, this site is methylated and inactive on the maternal homolog and unmethylated and transcriptionally active on the paternal homolog. In research samples from Prader-Willi individuals, only the methylated allele is present, while samples from Angelman Syndrome (AS) individuals contain only the unmethlyated allele. The CpG WIZ Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation -specific PCR (MSP). The kit contains primers targeted to the CpG island on the SNRPN gene. PCR parameters have been identified such that both primer sets in the kit amplify template under the same conditions. Normal control genomic DNA, which will generate a product with both primer sets, is also included.



Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

應用

Principles of the Technique

Use of either the CpGenome DNA Modification Kit (Cat. No. S7820) or the CpGENOME Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG Wiz Prader-Willi/Angelman Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
CPGWIZ is a trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

10 - Combustible liquids


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