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RSHMAG-69K

Millipore

MILLIPLEX® 大鼠应激激素磁珠面板-内分泌多重检测

This MILLIPLEX MAP Rat Stress Hormone Magnetic Bead Panel is an Endocrine multiplex assay. The analytes available for this multiplex kit are: ACTH, Corticosterone, Melatonin.

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84

品質等級

物種活性

rat

製造商/商標名

Milliplex®

assay range

accuracy: 61%
(Corticosterone)

accuracy: 83%
(ACTH)

sensitivity: 0.9 pg/mL
(ACTH)

sensitivity: 1.52 pg/mL
(Melatonin)

sensitivity: 2914 pg/mL
(Corticosterone)

standard curve range: 0.55-400 pg/mL
(Melatonin)

standard curve range: 1.4-1,000 pg/mL
(ACTH)

standard curve range: 686-500,000 pg/mL
(Corticosterone)

inter-assay cv: <15%
intra-assay cv: <10%

技術

multiplexing: suitable

檢測方法

fluorometric (Luminex xMAP)

運輸包裝

wet ice

一般說明

应激被定义为通过生理和/或心理刺激或应激源破坏体内稳态。在生理上,包括下丘脑、垂体和肾上腺在内的神经内分泌系统在应激反应中起关键作用。在促肾上腺皮质激素释放激素(CRH)和精氨酸加压素(AV)的激活下,垂体释放ACTH,导致肾上腺分泌皮质醇/皮质酮和其他糖皮质激素。这些皮质激素在应激反应中涉及全身,并通过对下丘脑的负反馈回路参与应激反应的终止。皮质酮和褪黑激素在调节睡眠-觉醒周期中很重要。

MILLIPLEX® 大鼠应激激素面板是一种3重试剂盒,用于同时定量血清、血浆、组织/细胞提取物或培养基样品中的以下任何或所有分析物:ACTH,皮质酮和/或褪黑激素。该试剂盒采用96孔板,包含冻干标准混合物、两个内部检测质控品,最多可测量38份样本,一式两份。

Luminex® xMAP®平台使用磁珠免疫分析格式,以实现理想的速度和灵敏度,同时定量多种分析物,从而显著提高生产力,同时节省有价值的样品量。

面板类型:内分泌

特異性

交叉反应性
此检测试剂在检测板内显示不显著的交叉反应性

應用

  • 分析物:ACTH、皮质酮、褪黑激素
  • 推荐样品类型:大鼠血清、血浆、细胞/组织上清液和裂解物
  • 注:为了获得浑浊的血清和血浆样品的最佳分析性能,提供了提取方案。
  • 建议的样品稀释度:每孔25 μL用于以下方面:未稀释的提取血清或血浆;1:4未提取的血清或血浆;细胞/组织培养样品可能需要在适当的对照培养基中稀释。
  • 分析运行时间:在2-8℃下过夜(16-18小时)
  • 研究类别:内分泌
  • 研究子类别:代谢

特點和優勢

通过在此面板中选择可用的分析物来设计多重试剂盒。

其他說明

请联系技术服务部了解稀释线性。

法律資訊

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

象形圖

Skull and crossbonesEnvironment

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


分析證明 (COA)

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存取文件庫

Jorge Moreno-Fernandez et al.
Nutrients, 11(3) (2019-03-17)
Although dietary iron is a determinant of iron status in animals, body fat mass has been reported to have an inverse association with iron status in human studies. The goal of this study was to determine the relationship between Fe
Chris P Jew et al.
PloS one, 8(8), e70415-e70415 (2013-08-14)
The group I metabotropic glutamate receptor 5 (mGluR5) has been implicated in the pathology of various neurological disorders including schizophrenia, ADHD, and autism. mGluR5-dependent synaptic plasticity has been described at a variety of neural connections and its signaling has been
Brooks A Gross et al.
Journal of neuroscience methods, 251, 83-91 (2015-05-28)
Sleep deprivation via gentle handling is time-consuming and personnel-intensive. We present here an automated sleep deprivation system via air puffs. Implanted EMG and EEG electrodes were used to assess sleep/waking states in six male Sprague-Dawley rats. Blood samples were collected
Brenda B García-Iglesias et al.
Neuropharmacology, 71, 216-227 (2013-04-02)
Serotonin (5-HT) modulates the hypothalamic-pituitary-adrenal (HPA) axis response to stress. We examined the effect of chronic restraint stress (CRS; 20 min/day) as compared to control (CTRL) conditions for 14 days, on: 1) restraint-induced ACTH and corticosterone (CORT) secretion in rats
Fernando Tadeu Serra et al.
Scientific reports, 9(1), 13684-13684 (2019-09-25)
Life experiences at early ages, such as physical activity in childhood and adolescence, can result in long-lasting brain effects able to reduce future risk of brain disorders and to enhance lifelong brain functions. However, how early physical exercise promotes these

相關內容

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

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