MMA205

Millicell^® µ-迁移检测试剂盒

This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear & lasts for more than 48 hours.

• 同義詞: microfluidic cellular migration assay kit
• 分類程式碼代碼: 12161503
• eCl@ss: 32161000
• NACRES: NB.13

屬性

• 物種活性: rat, mouse, human
• 製造商／商標名: Millicell^®
• 技術: cell based assay: suitable; single cell analysis: suitable
• 運輸包裝: dry ice

描述

一般說明

• Cell migration occurs in a variety of biological processes includingwound healing, embryonic development, and immune response. Because of the importance of cell migration in angiogenesis, oncology, neurology, andespecially immunology, there is an increased interest in understanding theunderlying biological mechanisms at a single cell level.
• Traditional multiwell (Boyden chamber) migration assays can have limitations, such as: (1) gradients are not linear, well-established, or controlled, (2) quantificationof migratory cells is based on an endpoint measurement rather than a dynamicdetermination, and (3) imaging of the cells while they are migrating iscurrently not possible.
• The Millicell µ-Migration Assay Kit overcomes these limitations.
• Its microfluidic, low-volume technology promotes a stable, diffusion-generatedconcentration gradient that is consistently linear and lasts for more than 48 hours. Designed for video microscopy assays, the µ-Migration Slide is made froma plastic with high optical qualities similar to those of glass. At specifictime intervals, images of the observation area can be acquired, allowingreal-time monitoring and quantitative measurements of cell migration.

特點和優勢

• Live cell tracking of single cell or cell population migration
• Establishes a stable and linear concentration gradient that lasts ≥ 48 hours
• Helps distinguish chemotaxis from random movement
• Allows for multiparametric analysis, including cell directionality and velocity
• Enhanced optical imaging of slow- and fast-migrating cells
• Analyze 3 chemoattractants in parallel for increased throughput and flexibility
• No need to spend time or money on optimization
• Ready to use, no assembly needed

成分

1. Millicell^® µ-迁移玻片：（部件号CS204500）4个无涂层灭菌玻片。每张玻片包含3个腔室。
2. Millicell^® µ-迁移帽和塞：（部件号CS204501）各48个。每个载玻片12个盖子和12个塞子。
3. 人IV型胶原: （部件号CS204498）一瓶100 µg的1 mg/mL人胶原IV。
4. 乙酸缓冲液：（部件号CS204485）一个装有1.5 mL 0.5 M乙酸的小瓶。
5. BSA溶液：（部件号CS204507）一个装有100 µL 30% BSA溶液的小瓶。
6. 斜面移液器吸头: Fisherbrand SureOne 200 µL吸头，Fisher目录号02-707-419。两个96头盒。

儲存和穩定性

1.Millicell^® µ-迁移玻片（部件号CS204500）。室温储存。
2.Millicellµ-迁移帽和插头（部件号CS204501）在室温下储存。
3.人IV型胶原（部件号CS204498）储存于-20ºC。
4.醋酸缓冲液（部件号CS204485）在室温下储存。
5.BSA溶液（部件号CS204507）储存于-20ºC。
6.斜面移液器吸头。室温储存。

法律資訊

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

安全資訊

• 儲存類別代碼: 10 - Combustible liquids