MABT913

Anti-Cytokeratin 19 Antibody, clone TROMA-3

clone TROMA-3, from rat

• 同義詞: Keratin, type I cytoskeletal 19, CK-19, Keratin-19, K19
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: TROMA-3, monoclonal
• 物種活性: mouse, human
• 包裝: antibody small pack of 25 μg
• 技術: electron microscopy: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_032497
• UniProt登錄號: P19001
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... KRT19(3880) ; mouse ... Krt19(16669)

描述

一般說明

Keratin, type I cytoskeletal 19 (UniProt: P19001; also known as Cytokeratin-19, CK-19, Keratin-19, K19) is encoded by the Krt19 (also known as Krt1-19) gene (Gene ID: 16669) in murine species. CK-19 belongs to the family of intermediate filament (IF) family, but differs from other IF proteins in lacking the C-terminal tail domain. About 20 different cytokeratin proteins have been reported in higher animals in varying combinations during various stages of development. CK-19 is normally expressed in the lining of the gastroenteropancreatic and hepatobiliary tracts. It is expressed throughout embryonic development with highest levels observed at 8.5 days post coitum. Its expression is shown to decrease by day 11.5 and then is increased again by 17.5 days post coitum. CK-19 is shown to be involved in the organization of myofibrils and together with keratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-19 has been shown to be an independent prognostic factor for pancreatic neuroendocrine tumors, especially the insulin-negative tumors. TROMA 3 has been found to stain trophoblastoma TDM.1 cells, a subpopulation of the retinoic acid.-induced F9 and PCC3/ND1 cells, and in 5-azacytidine-treated 1246 cells.

特異性

Clone TROMA-3 detects cytokeratin 19 in human and murine cells.

免疫原

Purified intermediate filaments from DM1 (Trophoblatoma) cell line.

應用

Anti-Cytokeratin 19, clone TROMA-3, Cat. No. MABT913, is a rat monoclonal antibody that detects Keratin, type I cytoskeletal 19 and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 19 in mouse lung tissue.

Western Blotting Analysis: A representative lot detected Cytokeratin 19 in Western Blotting applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Electron Microscopy Analysis: A representative lot detected Cytokeratin 19 in Electron Microscopy applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Immunofluorescence Analysis: A representative lot detected Cytokeratin 19 in Immunofluorescence applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Immunohistochemistry Analysis: A representative lot detected Cytokeratin 19 in Immunohistochemistry applications (Takase, H.M., et. al. (2013). Genes Dev. 27(2):169-81).

Immunoprecipitation Analysis: A representative lot detected Cytokeratin 19 in Immunoprecipitation applications (Ichinose, Y., et. al. (1990). Biochem Biophys Res Commun. 167(2):644-7).

品質

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytokeratin 19 in 10 µg of MCF-7 cell lysate.

標靶描述

~44 kDa observed; 44.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable