MABT857M

Anti-Muscle Satellite Cells Antibody, clone SM C-2.6

clone SM/C-2.6, from rat

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: SM/C-2.6, monoclonal
• 物種活性: mouse
• 包裝: antibody small pack of 25 μg
• 技術: flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable
• 同型: IgG1κ
• 目標翻譯後修改: unmodified

描述

一般說明

Skeletal muscle satellite cells are quiescent mononucleated myogenic cells that reside between the sarcolemma and basement membrane of terminally-differentiated muscle fibers. They serve as a reserve population of cells with stem-like properties. The number of satellite cells are shown to be highest in postnatal muscle, but declines with age. In their quiescent stage, satellite cells are rounded and mononucleated, and do not express relevant amounts of myogenic proteins. Also, in their quiescent state they are shown to be c-kit-, Sca-1-, CD34+, and CD45-. Satellite cells can proliferate in response to injury and regenerate muscle and produce more satellite cells. Hence, they have been considered to be the only myogenic source for the maintenance of skeletal muscle. When muscle regeneration is initiated, satellite cells changes from their quiescent state to an active stage and begin to proliferative. They can proliferate and then either fuse with each other to form myotubes, which mature into myofibers, or they can fuse with damaged segments of muscle fibers. (Ref.: Fukuda, S et al. (2004). Exp. Cell Res. 296(2); 245-255; Morgan JE and Partridge, TA (2003). Int. J. Biochem. Cell Biol. 35(8); 1151 1156).

特異性

Clone SM C-2.6 detects quiescent satellite cells from adult mouse muscle.

免疫原

C2/4 myogenic cells.

應用

Anti-Muscle Satellite Cells, clone SM C/2.6, Cat. No. MABT857, is a rat monoclonal antibody that detects muscle satellite cells and has been tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Fluorescence Activated Cell Sorting (FACS).

Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot detected Muscle Satellite Cells in Fluorescence Activated Cell Sorting (FACS) applications (Urbani, L., et. al. (2012). PLoS One. 7(11):e49860).

Flow Cytometry Analysis: A representative lot detected Muscle Satellite Cells in Flow Cytometry applications (Ikemoto, M., et. al. (2007). Mol Ther. 15(12):2178-85; Fukada, S., et. al. (2004). Exp Cell Res. 296(2):245-55).

Immunohistochemistry Analysis: A representative lot detected Muscle Satellite Cells in Immunohistochemistry applications (Fukada, S., et. al. (2004). Exp Cell Res. 296(2):245-55; Yao, Y., et. al. (2016). Nat Commun. 7:11415).

Immunofluorescence Analysis: A representative lot detected Muscle Satellite Cells in Immunofluorescence applications (Fukada, S., et. al. (2004). Exp Cell Res. 296(2):245-55).

Research Category
Cell Structure

品質

Evaluated by Flow Cytometry in C2C12 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Muscle Satellite Cells in a population of one million C2C12 cells.

外觀

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1