MABS2068

Anti-HSD3B1 Antibody, clone 4C1

clone 4C1, from mouse

• 同義詞: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, Trophoblast antigen FDO161G
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 4C1, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_000853.1
• UniProt登錄號: P14060
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HSD3B1(3283)

描述

一般說明

3 beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 1 (UniProt: P14060; also known as 3 beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type I, 3-beta-HSD I, Trophoblast antigen FDO161G) is encoded by the HSD3B1 (also known as 3BH, HSDB3A) gene (Gene ID: 3283) in human. HSD3B1 is a single -pass membrane protein localized in mitochondria and endoplasmic reticulum. Its transmembrane region is localized to amino acids 288-308. It is a bifunctional enzyme that catalyzes the oxidative conversion of Delta 5-ene-3-beta-hydroxy steroid and causes oxidative conversion of ketosteroids. It plays a vital role in the biosynthesis of all classes of hormonal steroids. Humans have two HSD3B isozymes. HSD3B1 is mainly expressed in the placenta, mammary and, prostate glands, and other peripheral tissues including the skin and adipose tissue and HSD3B2 is expressed primarily in the adrenal and gonads. They exhibit about 93% sequence homology. (Ref.: Gomez-Sanchez, CE et al. (2017). Steroids 127; 56-61).

特異性

Clone 4C1 detects 3-beta-HSD I (HSD3B1) in human tissues. It targets an epitope with in 14 amino acids from the N-terminal region.

免疫原

A KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human 3 beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 1 (HSD3B1).

Epitope: N-terminus

應用

Anti-HSD3B1, clone 4C1, Cat. No. MABS2068, is a mouse monoclonal antibody that detects 3-beta-HSD I (HSD3B1) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected HSD3B1 in human adrenal gland tissue sections.

ELISA Analysis : A representative lot detected HSD3B1 in ELISA applications (Gomez-Sanchez, C.E., et. al. (2017). Steroids. 127:56-61).

Western Blotting Analysis: 1 µg/mL from a representative lot detected HSD3B1 in human placenta tissue lysate in Western blotting applications.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected HSD3B1 in Immunohistochemistry applications (Gomez-Sanchez, C.E., et. al. (2017). Steroids. 127:56-61).

Western Blotting Analysis: A representative lot detected HSD3B1 in Western Blotting applications (Gomez-Sanchez, C.E., et. al. (2017). Steroids. 127:56-61).

Research Category
Signaling

品質

Evaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected HSD3B1 in human placenta tissue sections.

標靶描述

~38 kDa observed; 42.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.