MABN2642

Anti-Munc13-1 Antibody, clone 11B-10G

• 分類程式碼代碼: 12352203
• NACRES: NA.74

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 11B-10G, monoclonal
• 分子量: calculated mol wt 196.36 kDa; observed mol wt ~190 kDa
• 純化經由: using protein G
• 物種活性: rat
• 包裝: antibody small pack of 100 μL
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: N-terminal half
• Protein ID登錄號: NP_074052
• UniProt登錄號: Q62768
• 運輸包裝: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Unc13a(64829)

描述

一般說明

Protein unc-13 homolog A (UniProt: Q62768; also known as Munc13-1) is encoded by the Unc13a (also known as Unc13h1) gene (Gene ID: 64829) in rat. Munc13-1 is a large multifunctional peripheral membrane protein that is essential for synaptic vesicle fusion and neurotransmitter release. It is expressed in brain, with highest levels observed in the olfactory bulb, striatum, cerebral cortex, hippocampus, and cerebellum. Some expression has also been reported in pancreatic islet cells. Munc13-1 is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and also participates in the activity-dependent refilling of readily releasable vesicle pool. It contains a variable N-terminal region that contains a C2A domain and a calmodulin-binding region (CaMb) and a conserved C-terminal region that contains the C1, C2B, MUN and C2C domains. The C2A domain can form either a homodimer or a heterodimer with RIMs and provides a switch that controls neurotransmitter release and couples exocytosis to diverse forms of Rab3- and RIM-dependent presynaptic plasticity. The CaMb region is reported to mediate Ca2+-dependent short-term plasticity. The C1 domain mediates diacylglycerol and phorbol ester-dependent potentiation of release and the C2B domain acts as a Ca2+- and PIP2-dependent modulator of short-term plasticity. The MUN domain is highly elongated and is essential for opening syntaxin-1. In the presynaptic terminal, Munc13-1 is shown to form multiple and discrete supramolecular self-assemblies that serve as independent vesicular release sites by recruiting syntaxin-1. At the synaptic contacts in the active zone, Munc13-1 is essential for the formation of the syntaxin-1/Munc18-1 complex. This supramolecular assembly provides open syntaxin-1 molecules for use in the formation of active SNARE complexes and thereby specifies the site for synaptic vesicle docking, priming, and release. Munc3-1 is shown to be essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. (Ref.: Quade, B., et al. (2019). eLife 8; e42806; Sakamoto, H., et al. (2018). Nat. Neurosci. 21(1); 41-49).

特異性

Clone 11B-10G is a mouse monoclonal antibody that detects Munc-13-1 protein in rat. It targets an epitope within 70 amino acids from the N-terminal half.

免疫原

His-tagged recombinant fragment corresponding to the first 320 amino acids from rat Protein unc-13 homolog A (Munc13-1).

應用

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Munc13-1 in Rat brain tissue lysate.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected Munc13-1 in Immunocytochemistry applications (Sakamoto, H., et al. (2018). Nat Neurosci.;21(1):41-49).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Munc13-1, clone 11B-10G, Cat. No. MABN2642, is a mouse monoclonal antibody that detects Protein unc-13 homolog A and is tested for use in Immunocytochemistry and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable