MABN2268

Anti-LRRK1 Antibody, clone 12F2.1

clone 12F2.1, from mouse

• 同義詞: Leucine-rich repeat serine/threonine-protein kinase 1, Leucine-rich repeat kinase 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 12F2.1, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_078928
• UniProt登錄號: Q38SD2
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... LRRK1(79705)

描述

一般說明

Leucine-rich repeat serine/threonine-protein kinase 1 (UniProt: Q38SD2, Leucine-rich repeat kinase 1) is encoded by the LRRK1 (also known as KIAA1790) gene (Gene ID: 79705) in human. Leucine-rich repeat kinase 1 is a member of the ROCO family of protein kinases. Two isoforms of Leucine-rich repeat kinase 1 have been described that are generated by alternative splicing. It contains a ROC (Ras in complex proteins) domain together with a MAPKKK-like kinase domain and several protein protein interactions. It is reported to form a complex with activated EGFR via interaction with Grb2. Subsequently, it is internalized to early endosomes along with EGFR. Leucine-rich repeat kinase 1 also regulates EGFR transport from early to late endosomes and is involved in regulating the motility of EGF-containing early endosomes that is dependent on its kinase activity. Leucine-rich repeat kinase 1 is also reported to play a role in regulating bone mass and mice with defective gene are prone to osteoporosis, increased bone mineralization, and diminished bone resorption. (Ref.: Hanafusa, H et al. (2011). Nature Commun. 2, Article number: 158).

特異性

Clone 12F2.1 detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) in human. It targets an epitope within 86 amino acids from the internal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 86 amino acids from the internal region of human Leucine-rich repeat serine/threonine-protein kinase 1.

應用

Anti-LRRK1, clone 12F2.1, Cat. No. MABN2268, is a highly specific mouse monoclonal antibody that detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected LRRK1 in human cerebral cortex, human testis, and human pancreatic cancer tissues.

品質

Evaluated by Western Blotting in human hypothalamus tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected LRRK1 in 10 µg of human hypothalamus tissue lysate.

標靶描述

~200 kDa observed; 225.39 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: does not flash
• 閃點（°C）: does not flash