MABN1559

Anti-ATBF1 Antibody, clone 5A1.1

clone 5A1.1, from mouse

• 同義詞: Zinc finger homeobox protein 3, AT motif-binding factor 1, AT-binding transcription factor 1, Alpha-fetoprotein enhancer-binding protein, Zinc finger homeodomain protein 3, ZFH-3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 5A1.1, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_008816
• UniProt登錄號: Q15911
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ZFHX3(463)

描述

一般說明

Zinc finger homeobox protein 3 (UniProt: Q15911; also known as AT motif-binding factor 1, AT-binding transcription factor 1, ATBF1, Alpha-fetoprotein enhancer-binding protein, Zinc finger homeodomain protein 3, ZFH-3) is encoded by the ZFHX3 (also known as ATBF1) gene (Gene ID: 463) in human. ATBF1 contains 23 C2H2-type zinc fingers and 4 homeobox DNA-binding domains. It is shown to translocate from the cytoplasm to the nucleus following TGF-beta stimulation. It acts as a transcriptional regulator that can act as an activator or a repressor. It inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. In concert with SMAD-dependent TGF-beta signaling, it can repress the transcription of AFP via its interaction with SMAD2/3. ATBF1 is shown to regulate myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression. It is not found in normal gastric mucosa, but is found in gastric carcinoma cells and its expression is higher in ER-positive breast tumors than ER-negative breast tumors. It is shown to down-regulate the MUC5AC promoter in gastric cancer cells.

特異性

Clone 5A1.1 recognizes Zinc finger homeobox protein 3 (ATBF1) in human cells. It targets an epitope with in 87 amino acids from the C-terminal region. This antibody recognizes isoform of 306 and 404 kDa.

免疫原

GST/His-tagged recombinant fragment corresponding to 87 amino acids from the C-terminal region of human Zinc finger homeobox protein 3.

應用

Anti-ATBF1, clone 5A1.1, Cat. No. MABN1559, is a highly specific mouse monoclonal antibody that targets Zinc finger homeobox protein 3 and has been tested in Western Blotting.

品質

Evaluated by Western Blotting in SHSY-5Y cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ATBF1 in 10 µg of SHSY-5Y cell lysate.

標靶描述

~404 kDa observed; 404.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable