MABF2788

Anti-SARS-CoV-2 nucleocapsid Antibody, clone 7B10-3E4

• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 7B10-3E4, monoclonal
• 分子量: calculated mol wt 45.63 kDa; observed mol wt ~50 kDa
• 物種活性: virus
• 包裝: antibody small pack of 100 μg
• 技術: ELISA: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1κ
• UniProt登錄號: P0DTC9
• 運輸包裝: dry ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

SARS-CoV-2 Nucleoprotein (UniProt: P0DTC9; also known as N, Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 43740575) in SARS-CoV-2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. The N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. The RNA binding region of SARS-CoV-2 is localized to amino acids 41-186 and its dimerization domain is in amino acids 258-361. Lack of cysteine is one of the common features in nucleoprotein from different coronaviruses. The complete N protein of SARS-CoV and COV-2 is a highly basic with positive net charges and high pI (10.11). Its N-terminal region is more basic with positive charge, while the C-terminal region is acidic with negative charge. The middle region of nucleoprotein displays relatively higher hydrophobicity and the two termini display greater hydrophilicity. Nucleoprotein can undergo phosphorylation at multiple serines and threonines and some of these phosphorylation events are essential for RNA binding, oligomerization and localization to nucleoli. Phosphorylation is also required for recruitment of host RNA helicase DDX1 (DEAD-Box Helicase 1) that facilitates template readthrough and enables longer subgenomic mRNA synthesis. Clone 7B10-3E4 exhibits high reactivity with SARS-CoV-2 and weak reactivity with SARS-CoV-1.

特異性

Clone 7B10-3E4 is a mouse monoclonal antibody that detects nucleocapsid protein of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It targets an epitope within the N-terminal domain. It also displays weak reactivity with SARS-CoV-1.

免疫原

His-tagged full-length nucleocapsid protein from Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

應用

Quality Control Testing

Evaluated by Western Blotting in lysates from HEK293T cells expressing SARS-CoV-2 Nucleocapsid protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Nucleocapsid protein in lysates from HEK293T cells expressing SARS-CoV-2 Nucleocapsid protein, but not in lysates from the wild type HEK293T cells.

Tested Applications

Western Blotting Analysis: A 1:20 dilution from a representative lot detected SARS-CoV-2 nucleocapsid protein in Whole cell lysates of HEK293T cells expressing the nucleocapsid (NC) proteins of various human coronaviruses (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

ELISA Analysis: A 1:3 dilution from a representative lot detected SARS-CoV-2 nucleocapsid protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SARS-CoV-2 nucleocapsid protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Immunofluorescence Analysis: A 1:5 dilution from a representative lot detected SARS-CoV-2 nucleocapsid in HeLa cells transiently transfected with SARS-CoV-2 NC-P2A-GFP (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SARS-CoV-2 nucleocapsid, clone 7B10-3E4, Cat. No. MABF2788, is a mouse monoclonal antibody that detects SARS-CoV-2 Nucleoprotein and is tested for use in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% Sodium Azide without glycerol.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable