MABF2117

Anti-insulin peptide–MHC Antibody, clone mAb287

clone mAb287, from mouse

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: mAb287, monoclonal
• 物種活性: mouse
• 包裝: antibody small pack of 25 μg
• 技術: ELISA: suitable; flow cytometry: suitable; inhibition assay: suitable
• 同型: IgG1κ
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Ins1(16333)

描述

一般說明

Juvenile diabetes or insulin-dependent diabetes mellitus (IDDM) is considered as an autoimmune disease where islet infiltration of T cells, B cells, macrophages, and dendritic cells leads to their progressive destruction. It is reported that non-obese diabetic mice (NOD) express a single MHC class II molecule, IAg7, which is shown to be essential for the development of IDDM. It is shown that the major T cell insulin epitope resides with in amino acids 9-23 of the beta chain (B:9-23) and this peptide can bind to IAg7 in multiple positions (registers). Pathogenic CD4 T cells can recognize beta 9-23:I Ag7 complex when the insulin peptide is bound in register 3 (R3), which places amino acids 14-22 of the beta chain in the core P1 to P9 position and places arginine at P9 position, which is highly unfavored for the IAg7 pocket. The binding motifs of IAg7 are degenerate and contain small hydrophobic residues at P4 and P6 position. This monoclonal antibody (clone mAb287) selectively binds to B:9-23 when presented by IAg7 only in R3. It blocks the binding of IAg7-B:10-23 R3 tetramers to cognate T cells and impedes T cell responses to soluble B:9-23 peptides, but does not affect recognition of any other peptide bound to IAg7. This antibody is also shown to diminish T cell infiltration in NOD mice, delay the development of IDDM and overt hyperglycemia. (Ref.: Stratmann, T., et al. (2000). J Immunol. 165(6); 3214-3225; Zhang, L., et al. (2014). Proc. Natl. Acad. Sci. USA. 111 (7); 2656-2661).

特異性

Clone mAb287 is a mouse monoclonal antibody that recognizes a molecular complex of mouse MHC class II (named I-Ag7) and a peptide derived from insulin B-chain, amino acids 9-22.

免疫原

Soluble I-Ag7 bearing Insulin R3 peptides.

應用

Anti-insulin peptide MHC, clone mAb287, Cat. No. MABF2117, is a mouse monoclonal antibody that detects Insulin peptide -MHC and has been tested for use in ELISA, Flow Cytometry, and Inhibition assay.

Flow Cytometry Analysis: A representative lot detected insulin peptide MHC in Flow Cytometry applications (Zhang, L., et. al. (2014). Proc Natl Acad Sci USA. 111(7):2656-61).

ELISA Analysis: A representative lot detected insulin peptide MHC in ELISA applications (Zhang, L., et. al. (2014). Proc Natl Acad Sci USA. 111(7):2656-61).

Inhibition Analysis: A representative lot of this antibody inhibited in vitro responses of insulin-specific T-cell hybridomas/transfectomas. (Zhang, L., et. al. (2014). Proc Natl Acad Sci USA. 111(7):2656-61).

Research Category
Signaling

品質

Evaluated by Isotype testing,

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.