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重要文件

04511

Supelco

活/死细胞双染色试剂盒

suitable for fluorescence

同義詞:

用于活/死细胞染色

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.32

適合性

suitable for fluorescence

品質等級

儲存溫度

−20°C

相關類別

應用

该活/死细胞复染试剂盒可同时对活细胞和死细胞进行荧光染色。该试剂盒包含钙黄绿素-AM和碘化丙啶(PI)两种溶液,分别用于染色活细胞和死细胞。其中钙黄绿素-AM,即钙黄绿素的乙酰羟甲基酯,具有高度亲脂性和细胞膜透过性。尽管钙黄绿素-AM自身并非荧光分子,但活细胞中的酯酶可以催化钙黄绿素-AM生成钙黄绿素,从而发出强烈的绿色荧光(λex 490 nm, λem 515 nm). 因此钙黄绿素-AM只能染色活细胞。而核染色染料PI不能透过活细胞的细胞膜。但可以透过死细胞膜的变性区域来到达细胞核,并与细胞中的DNA双螺旋结构结合,从而发出红色荧光(λex 535 nm, λem 617 nm)。钙黄绿素和PI-DNA都能被490nm波长的光激发,因此可以采用荧光显微镜同时监测活细胞和死细胞。使用λex激发光时只能观察到死亡细胞。

僅套裝組件

產品號碼
描述

  • Solution A (Calcein AM solution) 4 × 50

  • Solution B (propidium iodide solution) 300 μL

相關產品

產品號碼
描述
訂價

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

185.0 °F - closed cup

閃點(°C)

85 °C - closed cup


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To investigate the presence and distribution of advanced glycation end products (AGE) in pulmonary fibrosis. Lung tissue samples obtained from seven necropsy cases with idiopathic pulmonary fibrosis and seven with normal pulmonary parenchyma were examined immunohistochemically with a monoclonal antibody
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The recent immunological demonstration of advanced glycation end products (AGE) of the Maillard reaction in several human tissues suggests a possible involvement of AGE in the aging process. We previously prepared a monoclonal anti-AGE antibody (6D12) which recognized N epsilon-(carboxymethyl)lysine.
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Studies of antimycotic host defenses have been limited by the paucity of rapid, reproducible quantitative assays for fungal cell damage. Prior studies defined a colorimetric method that uses MTT, a tetrazolium dye, to quantify polymorphonuclear leukocyte (PMNL)-mediated damage to fungi.
Elea Boucard et al.
Frontiers in bioengineering and biotechnology, 10, 920929-920929 (2022-08-09)
In tissue engineering, cell origin is important to ensure outcome quality. However, the impact of the cell type chosen for seeding in a biocompatible matrix has been less investigated. Here, we investigated the capacity of primary and immortalized fibroblasts of
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Clinical nephrology, 49(5), 273-280 (1998-06-09)
Cardiovascular disease is one of the most common complications of dialysis and renal transplant patients, and high levels of AGE are present in end-stage renal failure. To address the potential involvement of AGE and growth factors in the pathophysiology of

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