MABF2008

Anti-IL-17F Antibody, clone MM17F-8F5

• 同義詞: Interleukin-17F
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: MM17F-8F5, monoclonal
• 分子量: calculated mol wt 17.94 kDa; observed mol wt ~15 kDa
• 純化經由: using protein G
• 物種活性: mouse
• 包裝: antibody small pack of 100 μg
• 技術: ELISA: suitable; flow cytometry: suitable; inhibition assay: suitable; neutralization: suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: Unknown
• Protein ID登錄號: NP_665855
• UniProt登錄號: Q7TNI7
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Il17f(257630)

描述

一般說明

Interleukin-17F (UniProt: Q7TNI7; also known as IL-17F) is encoded by the Il17f gene (Gene ID: 257630) in murine species. The IL-17 family of cytokines contains six members that share four conserved cysteine residues at their C-terminal region that participate in the formation of intermolecular disulfide linkages. Members of this family are associated with the pathogenesis of multiple autoimmune diseases including rheumatoid arthritis, multiple sclerosis, and inflammatory bowel diseases. IL-17F is an effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. It is a disulfide-linked homodimeric protein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to produce mature secreted protein (aa 29-161). It can also form a heterodimer with IL-17A. Its actions are mediated via interaction with IL-17RA and IL-17RC receptors with 2:1 binding stoichiometry. It is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation. As signature effector cytokine of T-helper 17 cells (Th17), it induces neutrophil activation and recruitment at infection and inflammatory sites. It is also known to stimulate the production of antimicrobial β-defensins by mucosal epithelial cells thereby limiting the entry of microbes through the epithelial barriers. (Ref.: St. Leger, AJ., et al. (2017). Immunity 47(1); 148 158; Chang, SH., and Dong, C. (2009). Cytokine.46(1); 7-11).

特異性

Clone MM17F-8F5 is a mouse monoclonal antibody that detects Interleukin-17F.

免疫原

Ovalbumin-conjugated, full-length recombinant murine IL-17F.

應用

Quality Control Testing

Evaluated by Western Blotting with recombinant mouse IL-17F.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant mouse IL-17F.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Interleukin-17F in Flow Cytometry application (St. Leger, A.J., et al. (2017). Immunity. 47(1):148-158.e5).

ELISA Analysis: Various dilutions of this antibody detected recombinant mouse IL-17F.

Neutralizing: A representative lot neutralized IL-17F activity and reduced neutrophil chemotactic activity produced by IL-17A / splenocytes activated under Th17 conditions.(Uyttenhove, C., et al. (2011). J Leukoc Biol. 89(6):1001-7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重構

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable