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Key Documents

MABF141B

Sigma-Aldrich

Anti-MHC class II (I-A/I-E), violetFluor® 450, clone M5/114 .15.2 Antibody

clone M5/114.15.2, from rat, violetFluor® 450

同義詞:

H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain (Ii), MHC class II-associated invariant chain, CD74, MHC class II (I-A/I-E)

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rat

品質等級

共軛

violetFluor® 450

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

M5/114.15.2, monoclonal

物種活性

mouse

包裝

antibody small pack of 25 μg

技術

flow cytometry: suitable

同型

IgG2bκ

UniProt登錄號

目標翻譯後修改

unmodified

基因資訊

mouse ... Cd74(16149)

一般說明

The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

免疫原

Immunogen information for this antibody is proprietary information.

應用

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-MHC class II (I-A/I-E) Antibody, violetFluor® 450, clone M5/114 .15.2, Cat. No. MABF1413 is validated for use in Flow Cytometry, for the detection of MHC class II (I-A/I-E).

品質

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected MHC class II (I-A/I-E) in one million C57Bl/6 splenocytes.

外觀

Purified by size-exclusion chromatography
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

violetFluor is a registered trademark of Tonbo Biotechnologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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